Elsayim Rasha, Alkhulaifi Manal M, Aloufi Abeer S, Felemban Razaz Abdulaziz, Eltayeb Lienda Bashier, Mohamed Asawir Esamaldeen Ebrahim, Alshammari Hanan O, Abudouleh Esra'a
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Int J Mol Sci. 2025 Apr 16;26(8):3765. doi: 10.3390/ijms26083765.
Pregnant women are at an increased risk of severe influenza complications, necessitating vaccination as a preventive measure. Despite World Health Organization (WHO) recommendations for influenza vaccination during pregnancy, vaccination rates remain suboptimal in many regions. This study aims to identify key differentially expressed genes (DEGs) and biological pathways modulated by influenza vaccination in pregnant women pre- and post-vaccination, contributing to improved vaccine strategies. Microarray data from gene expression omnibus GEO dataset GSE166545 was analyzed to identify DEGs in blood samples from pregnant women at three time points: pre-vaccination (Day 0) and post-vaccination (Days 0 and 1) (Days 1 and 7). DEGs were filtered using an adjusted -value < 0.05 and |log2 fold change| ≥ 1. Protein/protein interaction (PPI) networks, hub gene identification, and pathway enrichment analyses were conducted using STRING, Cytoscape, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome databases. Hub gene validation was performed using the Human Protein Atlas (HPA) and GTEx Portal. The GSE166545 dataset analysis revealed 60 up-regulated and 12,854 down-regulated genes (Day 1 vs. 7), 55 up-regulated and 12,933 down-regulated genes (Day 0 vs. 1), and two up-regulated with no down-regulated genes (Day 0 vs. 7). Key pathways included interferon alpha/beta (IFN-γ\ β) signaling and toll-like receptor signaling (TLR). Hub genes such as GBP1, CXCL10, RSAD2, and IFI44 demonstrated robust up-regulation, correlating with enhanced immune responses. The initial observation of JCHAIN's notable up-regulation occurred on the seventh day following vaccination. Validation confirmed these genes' roles in antiviral defense mechanisms and vaccine responses. The findings reveal distinct immune response dynamics in pregnant women following influenza vaccination, highlighting potential biomarkers for vaccine efficacy. This study underscores the importance of tailored vaccine strategies to improve maternal and neonatal outcomes.
孕妇患严重流感并发症的风险增加，因此需要接种疫苗作为预防措施。尽管世界卫生组织（WHO）建议在孕期接种流感疫苗，但许多地区的接种率仍不理想。本研究旨在确定孕妇接种流感疫苗前后关键的差异表达基因（DEG）和受其调节的生物学途径，以改进疫苗策略。分析了来自基因表达综合数据库GEO数据集GSE166545的微阵列数据，以确定孕妇血液样本在三个时间点的DEG：接种前（第0天）以及接种后（第0天和第1天）（第1天和第7天）。使用校正后的P值<0.05和|log2倍数变化|≥1对DEG进行筛选。使用STRING、Cytoscape、京都基因与基因组百科全书（KEGG）和Reactome数据库进行蛋白质/蛋白质相互作用（PPI）网络分析、枢纽基因鉴定和通路富集分析。使用人类蛋白质图谱（HPA）和GTEx门户进行枢纽基因验证。GSE166545数据集分析显示，（第1天与第7天）有60个基因上调，12854个基因下调；（第0天与第1天）有55个基因上调，12933个基因下调；（第0天与第7天）有2个基因上调，无基因下调。关键途径包括干扰素α/β（IFN-γ/β）信号传导和Toll样受体信号传导（TLR）。GBP1、CXCL10、RSAD2和IFI44等枢纽基因表现出强烈上调，与增强的免疫反应相关。JCHAIN显著上调的最初观察结果出现在接种疫苗后的第7天。验证证实了这些基因在抗病毒防御机制和疫苗反应中的作用。研究结果揭示了孕妇接种流感疫苗后独特的免疫反应动态，突出了疫苗效力的潜在生物标志物。本研究强调了制定个性化疫苗策略以改善母婴结局的重要性。
Zotero 插件