Park Beamjun, Lee Soohyeon, Jo Inyoung, Kang Donghyun, Kim Taewan, Ryu Jaesung, Kong Hyejeong, Baek Moojun, Ahn Taesung
Department of Medical Life Science, Soonchunhyang University, Asan 31538, Republic of Korea.
Department of Colorectal Surgery, College of Medicine, Soonchunhyang University Cheonan Hospital, Cheonan 31151, Republic of Korea.
Int J Mol Sci. 2025 Apr 18;26(8):3849. doi: 10.3390/ijms26083849.
Radiotherapy is a cornerstone of colorectal cancer (CRC) treatment; however, its therapeutic efficacy is often compromised by both intrinsic and acquired resistance in CRC cells. This study employed small interfering RNA (siRNA) technology to elucidate the functional roles of BAMBI, GADD34, NFKBIA, and NFKBID in CRC cell lines SW480 and HCT116. We assessed their impact on key cellular processes and radiation sensitivity. Gene silencing of all four target genes significantly suppressed CRC cell proliferation, migration, and invasion. Moreover, siRNA-mediated knockdown enhanced radiation sensitivity, as evidenced by a substantial increase in apoptosis and a marked reduction in cell viability compared with controls. These findings suggest that BAMBI, GADD34, NFKBIA, and NFKBID serve as critical regulators of CRC progression and radiation resistance. Overall, this study provides a mechanistic foundation for further exploration into the pathways underlying radiation resistance and underscores the potential for developing personalized radiotherapy strategies guided by molecular profiling.
放射治疗是结直肠癌（CRC）治疗的基石；然而，其治疗效果常常受到CRC细胞内在和获得性耐药的影响。本研究采用小干扰RNA（siRNA）技术来阐明BAMBI、GADD34、NFKBIA和NFKBID在CRC细胞系SW480和HCT116中的功能作用。我们评估了它们对关键细胞过程和放射敏感性的影响。所有四个靶基因的基因沉默均显著抑制了CRC细胞的增殖、迁移和侵袭。此外，与对照组相比，siRNA介导的敲低增强了放射敏感性，表现为凋亡显著增加和细胞活力显著降低。这些发现表明，BAMBI、GADD34、NFKBIA和NFKBID是CRC进展和放射抗性的关键调节因子。总体而言，本研究为进一步探索放射抗性的潜在机制提供了理论基础，并强调了开发以分子谱分析为指导的个性化放疗策略的潜力。
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