Xu Xin, Gao Yaxin, Ren Shuanghui, Liu Zhongwen, Zhang Yongjun, Zhang Zhen, Lian Yanxian, Zhu Xuwei
Henan Sericultural Research Institute, Zhengzhou 450008, China.
Henan Agricultural University College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450008, China.
Insects. 2025 Mar 21;16(4):333. doi: 10.3390/insects16040333.
This experiment investigated the role of the gut microbiota in the growth and development of , isolated gut bacteria related to lignin degradation in oak trees, and preliminarily verified their lignin degradation ability, providing a new tool for the field of biodegradation. The changes in the structure of the gut microbial community of the oak silkworm at different developmental stages were analyzed via high-throughput sequencing of the 16SrDNA genes of the gut microbiota of fifth-instar larvae. A series of biological analyses were conducted, including amplicon sequence variant (ASV), microbial community diversity, microbial community composition, species difference, and evolutionary analyses. , , , , , , and were the dominant bacterial groups in the gut microbiota of the larvae, with comprising the highest proportion, thereby providing a reference basis for a deeper understanding of the functions of the gut microbiota in insect growth and development. In this study, we activated and identified the MYZ028 strain isolated from the fifth-instar larvae of through molecular biology, and we determined that the strain belonged to Bacillus subtilis. In terms of performance, the strain exhibited a high lignin-degrading ability and could effectively degrade lignin in a short time and simple environment. Due to the complex and difficult nature of degrading the lignin structure, research on the degradation of lignin by composite strains can be explored in the field of biodegradation.
本实验研究了肠道微生物群在柞蚕生长发育中的作用，分离出与柞树木质素降解相关的肠道细菌，并初步验证了它们的木质素降解能力，为生物降解领域提供了一种新工具。通过对五龄幼虫肠道微生物群的16SrDNA基因进行高通量测序，分析了柞蚕在不同发育阶段肠道微生物群落结构的变化。进行了一系列生物学分析，包括扩增子序列变体（ASV）、微生物群落多样性、微生物群落组成、物种差异和进化分析。芽孢杆菌属、肠杆菌属、假单胞菌属、葡萄球菌属、沙雷氏菌属、寡养单胞菌属和伯克霍尔德氏菌属是幼虫肠道微生物群中的优势细菌类群，其中芽孢杆菌属占比最高，从而为更深入了解肠道微生物群在昆虫生长发育中的功能提供了参考依据。在本研究中，我们通过分子生物学方法激活并鉴定了从柞蚕五龄幼虫中分离出的MYZ028菌株，确定该菌株属于枯草芽孢杆菌。在性能方面，该菌株表现出较高的木质素降解能力，能够在短时间和简单环境中有效降解木质素。由于木质素结构降解的复杂性和困难性，复合菌株降解木质素的研究可在生物降解领域进行探索。
