Gu Li, Qian Sheng, Yao Shuting, Wu Jiaxin, Wang Lianghong, Mu Jing, Wang Yankun, Wang Jianming, Zhang Zhongyi, Li Mingjie
College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Pathogens. 2025 Apr 5;14(4):353. doi: 10.3390/pathogens14040353.
(Miq.) Pax () was a valued traditional Chinese herbal medicine. Previous studies have shown that TuMV spreads during the vegetative propagation cycle using tuberous roots as carriers. However, the transmission mechanism of TuMV in and its effects on host growth remain to be elucidated. In this study, virus-free culture seedlings were infected with control, TuMV-ZR, and TuMV-ZR-EGFP, thereby resulting in the initial infection cycle of IF1 (TIF1, TEIF1) and control NIF1, and used these roots to propagate the subsequent infection cycle IF2 (TIF2, TEIF2) and control NIF2. The transmission of TuMV-ZR seedlings was tracked by EGFP signal, and their yield, quality, and resistance were analyzed simultaneously in the critical growth period of the plants. The results indicated that TuMV-ZR accumulated in the tuberous roots of IF1 plants, subsequently migrated to IF2 during seedling growth, and was re-stored in IF2 tuberous roots, thereby forming a simple virus transmission cycle. Meanwhile, the tuberous roots of IF1 and IF2 showed lower fresh weight, dry weight, soluble sugar, and saponin levels compared to NIF1 and NIF2, respectively. TuMV caused a significant reduction in chlorophyll synthesis in IF1 and IF2 , resulting in impairment to their photosynthetic organs and efficiency. The measurement of stress resistance in IF1 and IF2 revealed that continuous viral infection disrupted antioxidant enzyme activity, increased the content of MDA, enhanced the activity of PAL, and elevated the levels of intracellular osmotic substances in both propagation cycles. The findings indicated that the accumulation of the TuMV-ZR virus during two successive vegetative propagation cycles induced physiological stress, impaired photosynthesis, and caused progressive yield and quality decline with each cycle. This study systematically examined the impact of TuMV-ZR persistence during vegetative propagation on key physiological and biochemical indices in , providing critical data to clarify vegetative-propagation-mediated germplasm degradation.
（米q.）白藜芦醇（Pax）是一种珍贵的传统中草药。先前的研究表明，芜菁花叶病毒（TuMV）在营养繁殖周期中以块根为载体传播。然而，TuMV在白藜芦醇中的传播机制及其对宿主生长的影响仍有待阐明。在本研究中，将无病毒白藜芦醇培养幼苗分别用对照、TuMV-ZR和TuMV-ZR-EGFP感染，从而产生IF1（TIF1、TEIF1）的初始感染周期和对照NIF1，并利用这些根繁殖后续感染周期IF2（TIF2、TEIF2）和对照NIF2。通过EGFP信号追踪TuMV-ZR幼苗的传播情况，并在植物的关键生长时期同时分析其产量、品质和抗性。结果表明，TuMV-ZR在IF1植株的块根中积累，随后在幼苗生长期间迁移到IF2，并重新储存在IF2块根中，从而形成一个简单的病毒传播循环。同时，与NIF1和NIF2相比，IF1和IF2的块根鲜重、干重、可溶性糖和皂苷水平分别较低。TuMV导致IF1和IF2白藜芦醇中叶绿素合成显著降低，导致其光合器官和效率受损。对IF1和IF2白藜芦醇的抗逆性测定表明，连续的病毒感染扰乱了抗氧化酶活性，增加了丙二醛（MDA）含量，增强了苯丙氨酸解氨酶（PAL）活性，并在两个繁殖周期中提高了细胞内渗透物质的水平。研究结果表明，在连续两个营养繁殖周期中TuMV-ZR病毒的积累诱导了生理胁迫，损害了光合作用，并导致每个周期产量和品质逐渐下降。本研究系统地研究了营养繁殖期间TuMV-ZR持续存在对白藜芦醇关键生理生化指标的影响，为阐明营养繁殖介导的种质退化提供了关键数据。
