Jinno Chiyo, Fujisaki Ken, Yotsui Izumi, Ouchi Motoki, Singh Prerna, Naramoto Satoshi, Takezawa Daisuke, Sakata Yoichi, Fujita Tomomichi
Faculty of Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.
Department of Bioscience, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
Sci Adv. 2025 May 9;11(19):eadr8298. doi: 10.1126/sciadv.adr8298. Epub 2025 May 7.
Cell-to-cell communication is essential for multicellular organisms. Plasmodesmata (PD) are plant-specific nanopore structures pivotal for cell-to-cell communication and plant survival. However, how PD form and their structure, regulation, and evolution remain largely unknown. Here, we demonstrate that the exogenous supply of abscisic acid (ABA), a well-conserved phytohormone in land plants, reduces primary PD density in the moss . This regulation requires all core components of the ABA signaling pathway. Furthermore, we reveal that ABA-INSENSITIVE 5, a well-conserved transcription factor in the ABA signaling pathway of land plants, plays a pivotal role in PD density regulation, whereas ABA-INSENSITIVE 3 does not. Our findings show that the ABA-induced reduction in primary PD density is mediated by these ABA-responsive factors in . Considering previous reports on ABA-dependent PD regulation in both moss and angiosperms, we propose that the ABA-mediated control of PD biogenesis and permeability represents a conserved mechanism in land plants, with critical implications for cell-to-cell communication and stress adaptation.
细胞间通讯对于多细胞生物至关重要。胞间连丝（PD）是植物特有的纳米孔结构，对细胞间通讯和植物生存起着关键作用。然而，PD如何形成及其结构、调控和进化在很大程度上仍不清楚。在这里，我们证明了脱落酸（ABA）的外源供应，一种在陆地植物中保守的植物激素，会降低苔藓中的初级PD密度。这种调控需要ABA信号通路的所有核心组分。此外，我们揭示了ABA不敏感5，一种在陆地植物ABA信号通路中保守的转录因子，在PD密度调控中起关键作用，而ABA不敏感3则不然。我们的研究结果表明，ABA诱导的初级PD密度降低是由这些ABA响应因子介导的。考虑到之前关于苔藓和被子植物中ABA依赖的PD调控的报道，我们提出ABA介导的PD生物发生和通透性控制是陆地植物中的一种保守机制，对细胞间通讯和胁迫适应具有重要意义。
