Xu Kedian, Liang Ruiqing, Ye Ziqing, Gao Yunling
State Key Laboratory of Advanced Separation Membrane Materials,College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
State Key Laboratory of Advanced Separation Membrane Materials,College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Nov 5;340:126321. doi: 10.1016/j.saa.2025.126321. Epub 2025 Apr 30.
A ratiometric and rapid-response fluorescent nanoprobe (NA-CQDs) for the detection of hypochlorous acid based on naphthalimide (NA) modified carbon quantum dots (CQDs) has been prepared. The nanoprobe NA-CQDs showed blue fluorescence with a unique yellow fluorescence in the presence of HClO, accompanying the emission wavelength change from 457 nm to 538 nm. The fluorescence detection limit for HClO was 99 nM with a linear range from 0.1 to 32 μM. The nanoprobe exhibited a rapid response to HClO within 20 s. The sensing mechanism might be attributed to the oxidation and cleavage of p-aminophenylether group on the nanoprobe NA-CQDs, leading to the FRET process from the CQDs to the NA due to enhanced ICT of the NA portion. A series of real applications were conducted including spiked real water samples, test paper strips, and fluorescence bioimaging in RAW 264.7 and HepG2 cells and nude mice, highlighting the potential application of NA-CQDs for future environmental sensing and biosensing in living things.
基于萘酰亚胺（NA）修饰的碳量子点（CQDs），制备了一种用于检测次氯酸的比率型快速响应荧光纳米探针（NA-CQDs）。纳米探针NA-CQDs在HClO存在下呈现蓝色荧光并伴有独特的黄色荧光，发射波长从457nm变为538nm。HClO的荧光检测限为99nM，线性范围为0.1至32μM。该纳米探针在20s内对HClO表现出快速响应。传感机制可能归因于纳米探针NA-CQDs上对氨基苯醚基团的氧化和裂解，由于NA部分的ICT增强，导致了从CQDs到NA的FRET过程。进行了一系列实际应用，包括加标实际水样、试纸条以及在RAW 264.7和HepG2细胞及裸鼠中的荧光生物成像，突出了NA-CQDs在未来环境传感和生物体内生物传感方面的潜在应用。
