Liu Yanbo, Zhang Zhaohan, Fang Yanbin, Song Yanfang, Li Jiannan, Feng Yujie
State Key Laboratory of Urban-rural Water Resource and Environment, Harbin Institute of Technology, No73, Huanghe Road, Nangang District, Harbin 150090, China.
State Key Laboratory of Urban-rural Water Resource and Environment, Harbin Institute of Technology, No73, Huanghe Road, Nangang District, Harbin 150090, China.
J Hazard Mater. 2025 Aug 15;494:138459. doi: 10.1016/j.jhazmat.2025.138459. Epub 2025 May 2.
Phenol containing wastewater (PCW) is highly toxic and difficult to be treated by traditional methods. This study utilized granular activated carbon (GAC) and Fe (Sponge iron) &GAC (Fe&G) in a laboratory-scale UASB reactor to mitigate the toxicity of phenol containing simulated wastewater (PCSW) and enhance treatment performance. Compared with GAC, Fe&G mediators achieves approximately 7 % and 24 % higher removal rates for COD and phenolic compounds, respectively. The methane accumulation in Fe&G group was about 10 % higher than that in GAC group and 22 % higher than that in blank group. Microbial analysis showed that compared with GAC, Fe&G mediators could enrich Petronas and Methanothrix to intensify Direct Interspecies Electron Transfer (DIET) to augment PCSW treatment and boost methane production. PICRUSt analysis showed that these mediators enriched key genes such as TCA cycle and CO methanogenesis pathway to improve microbial resistance to PCSW toxicity and enhance microbial metabolism. This study provides a new method for anaerobic treatment of highly polluted industrial wastewater.
含酚废水毒性极强，难以用传统方法处理。本研究在实验室规模的UASB反应器中使用颗粒活性炭（GAC）以及铁（海绵铁）和GAC（Fe&G）来降低含酚模拟废水（PCSW）的毒性并提高处理性能。与GAC相比，Fe&G介质对化学需氧量（COD）和酚类化合物的去除率分别高出约7%和24%。Fe&G组的甲烷积累量比GAC组高约10%，比空白组高22%。微生物分析表明，与GAC相比，Fe&G介质可富集马来西亚石油公司菌属和甲烷丝状菌属，以强化种间直接电子传递（DIET），从而增强PCSW处理效果并提高甲烷产量。PICRUSt分析表明，这些介质富集了三羧酸循环和一氧化碳甲烷化途径等关键基因，以提高微生物对PCSW毒性的抗性并增强微生物代谢。本研究为高污染工业废水的厌氧处理提供了一种新方法。
