Tang Juan, Xiong Weijie, Liu Xianguo, Shi Yuhui, Yu Yanxin, Shi Maolin, Xu Hongyu
Department of Oncology, 363 Hospital, No. 108 Daosangshu Street, Wuhou District, Chengdu, 610041, Sichuan, China.
Department of Oncology, Chengdu Fifth People's Hospital, Affiliated Fifth People's Hospital of Chengdu University of Traditional Chinese Medicine, Cancer Prevention and Treatment Institute of Chengdu, Chengdu, China.
Discov Oncol. 2025 May 7;16(1):685. doi: 10.1007/s12672-025-02516-0.
Phloretin (Ph), an apple polyphenol, has been shown to possess anti-tumor effects. This study aimed to investigate the anti-tumor effects of the combination of Ph and radiotherapy on lung cancer.
The proliferative rate of Lewis lung carcinoma (LLC) cells treated with Ph was evaluated using the MTT assay. The radiosensitization effect of Ph was assessed using the clone formation assay. Additionally, the anti-tumor and radiosensitization effects of Ph were explored in LLC xenografts in mice.
Ph inhibited the proliferation of LLC cells in a time- and dose-dependent manner (p < 0.05). Moreover, the combination of Ph with radiotherapy significantly inhibited LLC cell colony formation (p < 0.05). In vivo studies demonstrated that the combination of Ph with radiotherapy significantly inhibited tumor growth, achieving a tumor inhibition rate of 74.44% compared to the control group (p < 0.01). This combination also prolonged the median survival times of mice by 31 days compared to the control group (p < 0.01), reduced tumor glucose uptake, promoted tumor cell apoptosis, and suppressed tumor cell proliferation.
This study suggests that the combination of Ph with radiotherapy exhibits promising activity against lung cancer, potentially through mechanisms including inhibition of glucose transport and promotion of apoptosis. These findings may provide a new therapeutic strategy for improving lung cancer treatment.
根皮素（Ph）是一种苹果多酚，已被证明具有抗肿瘤作用。本研究旨在探讨Ph与放疗联合应用对肺癌的抗肿瘤作用。
采用MTT法评估Ph处理的Lewis肺癌（LLC）细胞的增殖率。使用克隆形成试验评估Ph的放射增敏作用。此外，在小鼠LLC异种移植模型中探讨了Ph的抗肿瘤和放射增敏作用。
Ph以时间和剂量依赖性方式抑制LLC细胞的增殖（p<0.05）。此外，Ph与放疗联合应用显著抑制LLC细胞集落形成（p<0.05）。体内研究表明，Ph与放疗联合应用显著抑制肿瘤生长，与对照组相比，肿瘤抑制率达到74.44%（p<0.01）。与对照组相比，该联合治疗还使小鼠的中位生存时间延长了31天（p<0.01），降低了肿瘤葡萄糖摄取，促进了肿瘤细胞凋亡，并抑制了肿瘤细胞增殖。
本研究表明，Ph与放疗联合应用对肺癌具有有前景的活性，可能通过抑制葡萄糖转运和促进凋亡等机制实现。这些发现可能为改善肺癌治疗提供一种新的治疗策略。
