Cherepenko E I, Lebedev V R
Mol Biol (Mosk). 1985 May-Jun;19(3):629-35.
Reassociation kinetics of great wax moth--Galleria mellonella nuclear DNA short fragments was studied within the C0t values of 0.02 and 10(4). The least squares analysis of the experimental curve obtained revealed two components with C0t1/2 = 0.72 and C0t1/2 = 750 appropriately. The fraction of the component with C0t1/2 = 0.72 in the genome of great wax moth is 22% and that of the other one is 61%. There is also a rapid component which renatured at the C0t values less then 0,02. The fraction of this component in the genome is 17%. The great wax moth genome size determined kinetically is 4,83 X 10(8) base pairs. It was shown that the efficiency of DNA molecules network formation can qualify the genome studied as being a short or a long period of interspersion. On the basis that 80-90% of great wax moth DNA molecules participated in network formation which is inherent to the short period interspersion genomes, it was concluded that the great wax moth genome is of the Xenopus-like type.
在0.02至10⁴的Cot值范围内研究了大蜡螟（Galleria mellonella）核DNA短片段的复性动力学。对所得实验曲线进行最小二乘法分析，结果显示有两个组分，其Cot1/2分别为0.72和750。在大蜡螟基因组中，Cot1/2 = 0.72的组分所占比例为22%，另一个组分的比例为61%。还有一个快速复性组分，在Cot值小于0.02时复性。该组分在基因组中的比例为17%。通过动力学方法确定大蜡螟基因组大小为4.83×10⁸碱基对。结果表明，DNA分子网络形成效率可用于判断所研究的基因组是短散布还是长散布类型。基于80%至90%的大蜡螟DNA分子参与了短散布基因组所特有的网络形成，得出大蜡螟基因组属于非洲爪蟾样类型的结论。
