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[雀鳝基因组的形成(雀鳝目)]

[Formation of the genome of the alligator gar Lepisosteus osseus (Ganoidomorpha) genome].

作者信息

Vladychenskaia N S, Kedrova O S, Petrov N B

出版信息

Mol Biol (Mosk). 1983 Mar-Apr;17(2):373-82.

PMID:6855762
Abstract

Genome structure of the alligator gar was studied by means of a comparison of reassociation kinetics of short and long DNA fragments, an estimation of hyperchromicity of reassociated repetitive DNA as a function of fragments length, and length estimation of S1-resistant duplexes by gel filtration. It was shown that most of the repeated sequences in the alligator gar DNA are no less than 2000 b.p. long and weakly divergent. Little or no interspersion of unique and short repeated sequences were observed in this genome. No highly divergent repeats were found in the alligator gar genome.

摘要

通过比较短DNA片段和长DNA片段的复性动力学、估计复性重复DNA的增色效应与片段长度的函数关系以及通过凝胶过滤估计S1抗性双链体的长度,对雀鳝的基因组结构进行了研究。结果表明,雀鳝DNA中的大多数重复序列长度不少于2000个碱基对,且差异较小。在该基因组中未观察到独特序列和短重复序列的少量或无散布现象。在雀鳝基因组中未发现高度差异的重复序列。

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