Gul'tiaev A P, Zheltovskiĭ N V
Mol Biol (Mosk). 1985 May-Jun;19(3):784-90.
The interactions of amino acid esters with poly(A)x2poly(U) and poly(A)xpoly(U) have been investigated by means of thermal denaturation of these polynucleotides. The esters under consideration raised the melting point, revealing the preferable binding to helical polynucleotide structures. The melting point shifts demonstrate the following sequence of the stabilities of these complexes: Arg greater than Lys much greater than His greater than Met greater than Ser greater than Gly. The same stability order is observed when studying the polynucleotide renaturation in the presence of esters. This order coincides with that previously obtained for the nucleotide base--amino acid ester complexes excepting basic amino acid esters. The ester interactions with poly(A) and poly(U) also reveal the specificity of monomer--monomer interactions. Some dynamic contributions into the studied specificity are also discussed.
通过这些多核苷酸的热变性研究了氨基酸酯与聚（A）x2聚（U）和聚（A）x聚（U）的相互作用。所研究的酯提高了熔点，表明其优先结合螺旋状多核苷酸结构。熔点的变化表明这些复合物的稳定性顺序如下：精氨酸大于赖氨酸远大于组氨酸大于甲硫氨酸大于丝氨酸大于甘氨酸。在酯存在下研究多核苷酸复性时观察到相同的稳定性顺序。该顺序与先前获得的核苷酸碱基 - 氨基酸酯复合物的顺序一致，但碱性氨基酸酯除外。酯与聚（A）和聚（U）的相互作用也揭示了单体 - 单体相互作用的特异性。还讨论了对所研究特异性的一些动力学贡献。
