Permogorov V I, Fomenko T N, Semiletov Iu A, Shvachkin Iu P, Tiaglov B V
Mol Biol (Mosk). 1985 Nov-Dec;19(6):1514-8.
Model peptides--L-Arg-Gly-L-Arg, L-Arg-L-Tyr-L-Arg and L-Arg-L-Phe-L-Arg bind to different DNAs and synthetic polynucleotides and are found in the major groove of the double helix. Polynucleotide complexes containing L-Arg-Gly-L-Arg were studied in order to consider the influence of the arginine residues on the polynucleotide melting temperature. It was shown, that L-Arg-L-Tyr-L-Arg and L-Arg-L-Phe--L-Arg lowers the melting temperature in all polynucleotides studied. The dependence of the melting temperature of polynucleotide (DNA)--L-Arg-L-Tyr(L-Phe)-L-Arg complexes upon the polynucleotide GC-content has been detected. These effects reflect the intercalation of peptide tyrosyl (or phenylalanyl) residues into the double-stranded polynucleotide.
模型肽——L-精氨酸-甘氨酸-L-精氨酸、L-精氨酸-L-酪氨酸-L-精氨酸和L-精氨酸-L-苯丙氨酸-L-精氨酸与不同的DNA和合成多核苷酸结合，并存在于双螺旋的大沟中。为了研究精氨酸残基对多核苷酸解链温度的影响，对含有L-精氨酸-甘氨酸-L-精氨酸的多核苷酸复合物进行了研究。结果表明，L-精氨酸-L-酪氨酸-L-精氨酸和L-精氨酸-L-苯丙氨酸-L-精氨酸降低了所有研究的多核苷酸的解链温度。已检测到多核苷酸（DNA）-L-精氨酸-L-酪氨酸（L-苯丙氨酸）-L-精氨酸复合物的解链温度对多核苷酸GC含量的依赖性。这些效应反映了肽的酪氨酰（或苯丙氨酰）残基插入双链多核苷酸中。
