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绿茶提取物通过诱导miR-34a表达抑制PC3前列腺癌细胞的细胞增殖

Suppression of Cellular Proliferation in PC3 Prostate Cancer Cells by Green Tea Extract Through Induction of miR-34a Expression.

作者信息

Soghrati Salek Moalemi Seyedeh Fatemeh, Safari Fatemeh, Ahvati Hiva

机构信息

Department of Biology, Faculty of Science University of Guilan Rasht Iran.

School of Biology, College of Science University of Tehran Tehran Iran.

出版信息

Food Sci Nutr. 2025 May 6;13(5):e70215. doi: 10.1002/fsn3.70215. eCollection 2025 May.

DOI:10.1002/fsn3.70215
PMID:40336533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12055519/
Abstract

Prostate cancer (PC) ranks as the fifth major cause of cancer-related fatalities globally. Exploring new methods with high efficacy and low side effects by using new compounds is always desired. Tea is considered the second most commonly consumed beverage among the population of the world. Polyphenols (or catechins) in green tea play a significant role in cellular signaling pathways. Herein, we evaluate the effects of green tea extract on suppression of cellular proliferation through testing the expression of miR-34a in PC3 prostate cancer cells. In this respect, PC3 prostate cancer cells were cultured and treated with green tea extract for 48 h. By using the qRT-PCR method, the expression of miR-34a was analyzed. Moreover, the expression of key proteins to regulate cellular proliferation, such as prostate specific antigen (PSA), AKT, cyclin dependent kinase 1 (CDK1), cyclin B1, c-Myc, p53, and phospho-androgen receptor (p-AR) was evaluated by using western blot. Our results indicated the induction of miR-34a, p53, and the inhibition of cyclin B1, p-AR, CDK1, p-AKT, PSA, c-Myc, and p-CDK1. Our findings can be used to design anti-tumor regimens that utilize natural product ingredients. However, additional research will be needed to identify anticancer activities of green tea via miR-34a in prostate cancer cells.

摘要

前列腺癌（PC）是全球癌症相关死亡的第五大主要原因。人们一直期望通过使用新化合物探索高效低副作用的新方法。茶被认为是世界人口中第二大最常饮用的饮品。绿茶中的多酚（或儿茶素）在细胞信号通路中发挥重要作用。在此，我们通过检测PC3前列腺癌细胞中miR-34a的表达来评估绿茶提取物对细胞增殖抑制的影响。在这方面，培养PC3前列腺癌细胞并用绿茶提取物处理48小时。通过qRT-PCR方法分析miR-34a的表达。此外，使用蛋白质印迹法评估调节细胞增殖的关键蛋白的表达，如前列腺特异性抗原（PSA）、AKT、细胞周期蛋白依赖性激酶1（CDK1）、细胞周期蛋白B1、c-Myc、p53和磷酸化雄激素受体（p-AR）。我们的结果表明miR-34a、p53的诱导以及细胞周期蛋白B1、p-AR、CDK1、p-AKT、PSA、c-Myc和p-CDK1的抑制。我们的研究结果可用于设计利用天然产物成分的抗肿瘤方案。然而，还需要进一步的研究来确定绿茶通过miR-34a在前列腺癌细胞中的抗癌活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c9/12055519/78a95d0ac31f/FSN3-13-e70215-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c9/12055519/68b91cde2654/FSN3-13-e70215-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c9/12055519/51a5cd4c7a79/FSN3-13-e70215-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c9/12055519/642296ea6c47/FSN3-13-e70215-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c9/12055519/78a95d0ac31f/FSN3-13-e70215-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c9/12055519/68b91cde2654/FSN3-13-e70215-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c9/12055519/51a5cd4c7a79/FSN3-13-e70215-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c9/12055519/642296ea6c47/FSN3-13-e70215-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7c9/12055519/78a95d0ac31f/FSN3-13-e70215-g003.jpg

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Heliyon. 2022 Aug 11;8(8):e10081. doi: 10.1016/j.heliyon.2022.e10081. eCollection 2022 Aug.
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