Department of Biology, Faculty of Science, University of Guilan, Rasht 4193833697, Iran.
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran 1651153511, Iran.
J Tradit Chin Med. 2022 Oct;42(5):681-686. doi: 10.19852/j.cnki.jtcm.2022.05.002.
To evaluate anticancer efficacy of green tea extract (GTE) on PC3 prostate cancer cells.
By using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot methods, the expression of miR-195 and the epithelial to mesenchymal transition (EMT) markers such as E-cadherin and vimentin was analyzed.
Based on the results of 2D and 3D cell culture models, the inhibition of EMT and up regulation of miR-195 expression were detected.
Our findings will be helpful to design anti-tumor regimens with natural product original, and more studies will be required to identify the related mechanisms involving anticancer activities of green tea miRNAs.
评估绿茶提取物（GTE）对 PC3 前列腺癌细胞的抗癌功效。
采用实时定量聚合酶链反应（qRT-PCR）和 Western blot 方法分析 miR-195 的表达和上皮间质转化（EMT）标志物如 E-钙黏蛋白和波形蛋白的表达。
基于 2D 和 3D 细胞培养模型的结果，检测到 EMT 的抑制和 miR-195 表达的上调。
我们的研究结果将有助于设计具有天然产物原始特性的抗肿瘤方案，还需要更多的研究来确定涉及绿茶 miRNA 抗癌活性的相关机制。
