Gerstenfeld L C, Finer M H, Boedtker H
Mol Cell Biol. 1985 Jun;5(6):1425-33. doi: 10.1128/mcb.5.6.1425-1433.1985.
Phorbol-12-myristate-13-acetate (PMA), a potent tumor promoter, was shown to have opposite effects on the cellular morphology and steady-state levels of beta-actin mRNA in embryonic chicken muscle fibroblasts and sternal chondrocytes. When fibroblasts were treated with PMA, they formed foci of densely packed cells, ceased to adhere to culture plates, and had significantly reduced levels of beta-actin mRNA and protein. Conversely, when treated with PMA, floating chondrocytes attached to culture dishes, spread out, and began to accumulate high levels of beta-actin mRNA and proteins. In the sternal chondrocytes the stimulation of the beta-actin mRNA production was accompanied by increased steady-state levels of fibronectin mRNAs and protein. These alterations were concomitant with a fivefold reduction in type II collagen mRNA and a cessation in its protein production. After fibronectin and actin mRNAs and proteins reached their maximal levels, type I collagen mRNA and protein synthesis were turned on. Removal of PMA resulted in reduced beta-actin mRNA levels in chondrocytes and in a further alteration in the cell morphology. These observed correlations between changes in cell adhesion and morphology and beta-actin expression suggest that the effect of PMA on cell shape and adhesion may result in changes in the microfilament organization of the cytoskeleton which ultimately lead to changes in the extracellular matrix produced by the cells.
佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）是一种强效肿瘤促进剂，已被证明对胚胎鸡肌肉成纤维细胞和胸骨软骨细胞的细胞形态以及β-肌动蛋白mRNA的稳态水平具有相反的影响。当用PMA处理成纤维细胞时，它们形成紧密堆积细胞的集落，不再粘附于培养板，并且β-肌动蛋白mRNA和蛋白质水平显著降低。相反，当用PMA处理时，漂浮的软骨细胞附着于培养皿，铺展并开始积累高水平的β-肌动蛋白mRNA和蛋白质。在胸骨软骨细胞中，β-肌动蛋白mRNA产生的刺激伴随着纤连蛋白mRNA和蛋白质稳态水平的增加。这些改变伴随着II型胶原mRNA减少五倍及其蛋白质产生的停止。在纤连蛋白和肌动蛋白mRNA及蛋白质达到其最大水平后，I型胶原mRNA和蛋白质合成开启。去除PMA导致软骨细胞中β-肌动蛋白mRNA水平降低以及细胞形态的进一步改变。这些观察到的细胞粘附和形态变化与β-肌动蛋白表达之间的相关性表明，PMA对细胞形状和粘附的影响可能导致细胞骨架微丝组织的变化，最终导致细胞产生的细胞外基质发生变化。
