Sankaranarayanan K, Natarajan A T, Mullenders L H, van Rijn J L
Mutat Res. 1985 Sep;151(2):269-74. doi: 10.1016/0027-5107(85)90079-x.
The effects of sodium butyrate-mediated alterations in chromatin structure on the yields of X-ray-induced chromosomal aberrations were studied in human peripheral blood lymphocytes. Unstimulated (G0) lymphocytes were pre-treated with sodium butyrate (5 mM) for 24 h, X-irradiated and then stimulated to pass through the cell cycle. Cells in their first post-radiation metaphase were scored for chromosomal aberrations. In parallel biochemical experiments nucleoid sedimentation technique was used to examine the induction and repair of DNA-strand breaks. The results show that sodium butyrate pre-treatment leads to a significant increase in the frequencies of dicentrics and rings, but not of fragments. The data from biochemical studies suggest that the numbers and rates of repair of X-ray-induced DNA-strand breaks are the same in butyrate-treated and untreated cells. We therefore suggest that the observed effect is probably a consequence of butyrate-induced conformational changes in the chromatin of G0 lymphocytes.
在人外周血淋巴细胞中研究了丁酸钠介导的染色质结构改变对X射线诱导的染色体畸变产率的影响。未受刺激的（G0期）淋巴细胞用丁酸钠（5 mM）预处理24小时，进行X射线照射，然后刺激其通过细胞周期。对辐射后第一个中期的细胞进行染色体畸变评分。在平行的生化实验中，使用核仁沉降技术检测DNA链断裂的诱导和修复。结果表明，丁酸钠预处理导致双着丝粒和环状染色体的频率显著增加，但片段的频率没有增加。生化研究的数据表明，丁酸钠处理和未处理的细胞中X射线诱导的DNA链断裂的修复数量和速率相同。因此，我们认为观察到的效应可能是丁酸钠诱导G0期淋巴细胞染色质构象变化的结果。
