Tan Lei, Yang Yong, Huang Xiaojiu, Yuan Youqing, Wang Kaixin, Peng Xiaoye, He Yiyan, Wang Yijin, Lei Lei, Chen Yingyi, Duan Deyong, Wang Naidong, Yang Yi, Dai Feiyan, Huang Cuiqing, Wang Aibing
College of Animal Science and Technology, Yangtze University, Jingzhou, China.
Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan, China.
Front Vet Sci. 2025 Apr 23;12:1572729. doi: 10.3389/fvets.2025.1572729. eCollection 2025.
Pseudorabies virus (PRV) represents a considerable infectious threat to the swine industry in China and poses potential health risks to humans. However, there is a notable lack of specific antiviral agents aimed at combating PRV. Haspin is involved in histone phosphorylation during mitosis, while the role of swine Haspin in PRV infection has not been previously investigated. In the present study, we demonstrated that Haspin expression was significantly enhanced in response to PRV infection. Overexpression of the gene notably enhanced PRV infection, while genetic inhibition of gene resulted in a substantial reduction in viral infection. Further investigations indicated that the Haspin kinase inhibitor CHR-6494 effectively suppressed PRV infection in a concentration-dependent manner, primarily by inhibiting viral virus replication rather than interfering with the processes of binding, entry, or release. Additionally, treatment with CHR-6494 effectively restricted Herpes simplex virus type 1 infection in Vero cells. Collectively, these findings indicate that Haspin may serve as a novel therapeutic target for the management of infections caused by .
伪狂犬病病毒（PRV）对中国养猪业构成了相当大的感染威胁，并对人类健康构成潜在风险。然而，目前明显缺乏针对PRV的特异性抗病毒药物。Haspin参与有丝分裂期间的组蛋白磷酸化，而猪Haspin在PRV感染中的作用此前尚未得到研究。在本研究中，我们证明PRV感染可显著增强Haspin的表达。该基因的过表达显著增强了PRV感染，而该基因的基因抑制则导致病毒感染大幅减少。进一步的研究表明，Haspin激酶抑制剂CHR-6494以浓度依赖的方式有效抑制PRV感染，主要是通过抑制病毒复制，而非干扰结合、进入或释放过程。此外，用CHR-6494处理可有效限制Vero细胞中1型单纯疱疹病毒的感染。总的来说，这些发现表明Haspin可能是治疗由[病毒名称未明确]引起的感染的新治疗靶点。
