Tan Lei, Zhu Pei, Getu Zhaori, Yang Xi, Zheng Shiling, Duan Yuqing, Wang Jinping, Zhou Yi, Hu Yuyang, Wang Yijing, Yang Yuying, Zuo Mengting, Yao Jun
College of Animal Science and Technology, Yangtze University, Jingzhou, Hubei, China.
Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, China.
Front Vet Sci. 2025 Jun 16;12:1623545. doi: 10.3389/fvets.2025.1623545. eCollection 2025.
Pseudorabies (PR), an infectious disease caused by pseudorabies virus (PRV), has been responsible for substantial economic losses within the global swine industry. However, effective control measures and vaccines against PRV remain limited, thereby underscoring the necessity for the development of innovative antiviral agents targeting PRV. Nitazoxanide (NTZ) is an FDA-approved anthelminthic drug, has shown efficacy in inhibiting a variety of viral infections. This study aims to evaluate the antiviral properties of NTZ against PRV infection . The findings demonstrated that NTZ treatment significantly inhibited PRV infection in a dose-dependent manner in both PK15 and Vero cell lines, with the primary inhibitory effect occurring during the viral replication phase, rather than during the attachment, entry, or release phases of the virus. Subsequent RNA-Seq analysis revealed that cellular signaling pathways related to oxidative stress were implicated in the antiviral efficacy of NTZ against PRV infection. In conclusion, our findings implicate that NTZ effectively suppress PRV infection , suggesting its potential as a promising antiviral candidate for the clinical interventions in infections.
伪狂犬病（PR）是由伪狂犬病病毒（PRV）引起的一种传染病，给全球养猪业造成了巨大的经济损失。然而，针对PRV的有效控制措施和疫苗仍然有限，这凸显了开发针对PRV的新型抗病毒药物的必要性。硝唑尼特（NTZ）是一种经美国食品药品监督管理局（FDA）批准的驱虫药，已显示出对多种病毒感染具有抑制作用。本研究旨在评估NTZ对PRV感染的抗病毒特性。研究结果表明，在PK15和Vero细胞系中，NTZ处理均以剂量依赖性方式显著抑制PRV感染，主要抑制作用发生在病毒复制阶段而非病毒的附着、进入或释放阶段。随后的RNA测序分析表明，与氧化应激相关的细胞信号通路与NTZ对PRV感染的抗病毒功效有关。总之，我们的研究结果表明NTZ能有效抑制PRV感染，表明其作为一种有前景的抗病毒候选药物用于临床干预感染的潜力
