Yang Yuanyuan, Si Jinglei, Mo Jiayuan, Li Jin, Pan Bin, Pan Yi, Jiang Lihe, Wang Decai, Feng Xueping
School of Basic Medicine, Youjiang Medical University for Nationalities, Baise, China.
College of Animal Science & Technology, Guangxi University, Nanning, China.
Front Microbiol. 2025 Apr 23;16:1554537. doi: 10.3389/fmicb.2025.1554537. eCollection 2025.
Obesity is increasingly becoming a challenge with China's economic development. There is an urgent need to identify more affordable methods to combat this condition. (PS), a cost-effective herbal remedy widely used in China for treating inflammation and pain, shows potential in this regard. To investigate its anti-obesity mechanisms, we established a high-fat diet (HFD)-induced obesity model in mice. The obese mice subsequently received daily oral gavage of PS extract for 21 consecutive days. Upon the completion of the experiment, blood samples were collected to analyze lipid profiles, including total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL-C), and low-density lipoprotein (LDL-C). Abdominal adipose tissue was subjected to hematoxylin-eosin (HE) staining for histological analysis, while fecal samples underwent 16S rRNA sequencing to assess gut microbiota composition. Our findings revealed that PS supplementation significantly reduced body weight, lipid metabolism biomarkers, and adipocyte size. PS treatment also restored gut microbial diversity, with 19 specific microbial taxa and 25 KEGG pathways identified as potential mediators of its anti-obesity effects. Notably, PS modulated key obesity-associated gut microbiota, including , , , , , and . Serum metabolomics analysis implicated L-ascorbic acid, stevioside, allopurinol, and gingerol, along with amino acid and energy metabolism pathways, in the anti-obesity mechanism of PS. These results provide novel theoretical insights into the therapeutic potential of PS for obesity prevention and treatment.
随着中国经济的发展，肥胖问题日益严峻。迫切需要找到更经济实惠的方法来应对这一状况。（PS）是一种在中国广泛用于治疗炎症和疼痛的具有成本效益的草药疗法，在这方面显示出潜力。为了研究其抗肥胖机制，我们在小鼠中建立了高脂饮食（HFD）诱导的肥胖模型。肥胖小鼠随后连续21天每天接受PS提取物的口服灌胃。实验结束后，采集血样分析血脂谱，包括总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL-C）和低密度脂蛋白（LDL-C）。对腹部脂肪组织进行苏木精-伊红（HE）染色以进行组织学分析，同时对粪便样本进行16S rRNA测序以评估肠道微生物群组成。我们的研究结果表明，补充PS可显著降低体重、脂质代谢生物标志物和脂肪细胞大小。PS治疗还恢复了肠道微生物多样性，确定了19个特定微生物分类群和25条KEGG途径作为其抗肥胖作用的潜在介质。值得注意的是，PS调节了与肥胖相关的关键肠道微生物群，包括 、 、 、 、 和 。血清代谢组学分析表明，L-抗坏血酸、甜菊糖苷、别嘌呤醇和姜辣素以及氨基酸和能量代谢途径参与了PS的抗肥胖机制。这些结果为PS在肥胖预防和治疗方面的治疗潜力提供了新的理论见解。
