Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, China.
J Food Biochem. 2021 Apr;45(4):e13541. doi: 10.1111/jfbc.13541. Epub 2021 Feb 11.
The current study employed high-fat diet (HFD) induced murine model to assess the relationship between the lipid-lowering effect of aged citrus peel (chenpi) extract and the alterations of gut microbiota. The results showed that intake of chenpi extract for 12 week dose-dependently suppressed HFD-induced body weight, food intake, Lee's index, together with decreased the level of fasting blood glucose, total cholesterol, triglyceride, and low-density lipoprotein cholesterol. Moreover, chenpi extract administration up-regulated the abundance and diversity of fecal microbiota and down-regulated the ratio of Firmicutes-to-Bacteroidetes, which was characterized by the lower family of Lachnospiraceae, Helicobacteraceae, and Desulfovibrionaceae, and higher family of Bacteroidales_S24-7, Bacteroidaceae, Rikenellaceae, and Ruminococcaceae. Consistently, at the genus levels, chenpi extract treatment reversed the expansions of Helicobacter, Lachnospiraceae_UCG-006, and Desulfovibrio, while increased the abundance of Bacteroides, Rikenellaceae_RC9_gut_group, and Alistipes (belonging to Rikenellaceae family), Anaerotruncus and Odoribacter (belonging to Ruminococcaceae family), which were significantly negatively correlated with the levels of the serum lipid parameters. In conclusion, our findings indicated that anti-obesity ability of chenpi extract might be related to the improvement of gut microbiota imbalance. PRACTICAL APPLICATIONS: With the improvement of living standards, the incidence of metabolic diseases such as obesity, hypertension, and diabetes has increased significantly, and it has become a public health problem that seriously affects the health of the people. Chenpi contains a large amount of active ingredients, flavonoids, and other compounds, which can promote the absorption of the digestive system and have good effects on diseases such as the cardiovascular system. Our previous study has confirmed that the chenpi extract effectively regulated the glucose and lipid metabolism disorder induced by high-fat diet. However, it is not clear whether the effect is closely related to the improvement of gut microbiota. Accordingly, our result would provide a theoretical basis for future research on the relationship between obesity, chenpi extract, and gut microbiota, and support additional understanding of its potential anti-obesity effects.
本研究采用高脂饮食（HFD）诱导的小鼠模型，评估陈皮提取物的降脂作用与肠道微生物群改变之间的关系。结果表明，12 周陈皮提取物摄入呈剂量依赖性地抑制 HFD 诱导的体重、食物摄入、Lee 指数增加，同时降低空腹血糖、总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平。此外，陈皮提取物给药可增加粪便微生物群的丰度和多样性，并降低厚壁菌门与拟杆菌门的比值，其特征为lachnospiraceae、helicobacteraceae 和 desulfovibrionaceae 家族减少，bacteroidales_s24-7、bacteroidaceae、reichenellaceae 和 ruminococcaceae 家族增加。一致地，在属水平上，陈皮提取物处理逆转了 helicobacter、lachnospiraceae_ucg-006 和 desulfovibrio 的扩张，同时增加了 bacteroides、reichenellaceae_rc9_gut_group 和 alistipes（属于 reichenellaceae 科）、anaerotruncus 和 odoribacter（属于 ruminococcaceae 科）的丰度，这些与血清脂质参数水平呈显著负相关。总之，我们的研究结果表明，陈皮提取物的抗肥胖能力可能与改善肠道微生物群失衡有关。实际应用：随着生活水平的提高，肥胖症、高血压和糖尿病等代谢性疾病的发病率显著增加，已成为严重影响人民健康的公共卫生问题。陈皮含有大量的活性成分、类黄酮等化合物，能促进消化系统的吸收，对心血管系统等疾病有很好的疗效。我们之前的研究已经证实，陈皮提取物能有效调节高脂饮食引起的糖脂代谢紊乱。然而，其效果是否与肠道微生物群的改善密切相关尚不清楚。因此，我们的研究结果为今后研究肥胖症、陈皮提取物与肠道微生物群之间的关系提供了理论依据，支持了对其潜在抗肥胖作用的进一步认识。
