Chreim Sajida, Hosseini Seyed Masoud, Medlej Abdallah, Tarhini Mahdi
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Department of Research and Academic Affairs, Sheikh Ragheb Harb University Hospital, Lebanese University, Nabatieh, Lebanon.
Iran J Microbiol. 2025 Apr;17(2):261-267. doi: 10.18502/ijm.v17i2.18386.
Uropathogenic (UPEC) is a leading cause of urinary tract infections, which are a significant public health concern worldwide. Antibiotic resistance among UPEC isolates is an increasing challenge, necessitating a better understanding of the resistance patterns and underlying genetic mechanisms. This study examined the prevalence of antibiotic resistance phenotypes and the detection of specific resistance genes among patients with UPEC infections in Sheikh Ragheb Harb University Hospital in south Lebanon.
Antimicrobial resistance phenotype of 104 urine samples was tested to determine the resistance percentages for various antibiotics including ampicillin, gentamicin, ciprofloxacin, tetracycline, bactrim, meropenem, and imipenem using disk diffusion test. Additionally, molecular analysis like polymerase chain reaction (PCR) was performed to detect the presence of , and resistance genes.
The antimicrobial resistance testing revealed the following resistance percentages for various antibiotics: ampicillin (100%), gentamicin (15.38%), ciprofloxacin (34.61%), tetracycline (48.07%), bactrim (17.3%), meropenem (0.96%) and imipenem (0.96%). The analysis of resistance genes showed the presence of (7.96%), (0.96%), (20.19%), and (0.96%) genes, while the , and genes were not detected.
The high rates of antibiotic resistance observed, particularly to ampicillin and tetracycline, highlight the need for more judicious antibiotic use and the development of alternative treatment strategies to combat UPEC infections. These results can inform antimicrobial stewardship programs and guide the selection of appropriate empiric therapy for urinary tract infections.
尿路致病性大肠杆菌（UPEC）是尿路感染的主要病因，而尿路感染是全球重大的公共卫生问题。UPEC分离株中的抗生素耐药性是一个日益严峻的挑战，因此有必要更好地了解耐药模式及潜在的遗传机制。本研究调查了黎巴嫩南部谢赫·拉格布·哈尔布大学医院UPEC感染患者中抗生素耐药表型的流行情况以及特定耐药基因的检测情况。
采用纸片扩散法检测104份尿液样本的抗菌耐药表型，以确定对包括氨苄西林、庆大霉素、环丙沙星、四环素、复方新诺明、美罗培南和亚胺培南在内的多种抗生素的耐药百分比。此外，还进行了聚合酶链反应（PCR）等分子分析，以检测blaCTX-M、blaTEM和blaSHV耐药基因的存在情况。
抗菌耐药性检测显示，各种抗生素的耐药百分比如下：氨苄西林（100%）、庆大霉素（15.38%）、环丙沙星（34.61%）、四环素（48.07%）、复方新诺明（17.3%）、美罗培南（0.96%）和亚胺培南（0.96%）。耐药基因分析显示存在blaCTX-M（7.96%）、blaTEM（0.96%）、blaSHV（20.19%）和blaOXA-48（0.96%）基因，而blaNDM、blaKPC和blaVIM基因未被检测到。
观察到的高抗生素耐药率，尤其是对氨苄西林和四环素的耐药率，凸显了更合理使用抗生素以及开发替代治疗策略以对抗UPEC感染的必要性。这些结果可为抗菌药物管理计划提供参考，并指导尿路感染适当经验性治疗的选择。
