Kuntaman Kuntaman, Masfufatun Masfufatun, Wibisono Freshinta Jellia, Sarassari Rosantia, Setyorini Wahyu, Tania Putu Oky Ari, Shirakawa Toshiro
Department of Medical Microbiology, Faculty of Medicine, Universitas Wijaya Kusuma Surabaya, Surabaya, Indonesia.
Department of Biochemistry, Faculty of Medicine, Universitas Wijaya Kusuma Surabaya, Surabaya, Indonesia.
Open Vet J. 2025 May;15(5):2230-2237. doi: 10.5455/OVJ.2025.v15.i5.40. Epub 2025 May 31.
Foodborne pathogens, particularly antibiotic-resistant strains, pose a significant threat to public health globally.
To explore the emergence and spread of extended-spectrum beta-lactamase (ESBL)-producing in the food chain, focusing on broiler chickens in wet markets.
Specimens were inoculated into TBX medium supplemented with cefotaxime 4 ug/ml for selective isolation of ESBL-producing . Phenotypic and genotypic characterization of the isolates was performed, including species detection using PCR for identification with specific primers targeting the gene and a double diffusion synergy test for ESBL identification.
A total of 262/305 (86%) samples were confirmed positive for ESBL-producing from broiler chickens and free-range chickens. were mostly identified among ESBL-producing from broiler chickens (91%) or free-range chicken (100%), followed by 20% and 23%, respectively. There were no identified genes. Among 50 ESBL producers, (31; 17.4% from broiler chickens and 19; 22.6% from free-range chicken) were identified with a co-incidence of and .
There is an increased prevalence of ESBL-producing in environmentally contaminated foods. Hygiene and antibiotic use regulations for poultry farms should be improved. ESBL genes among chickens are mainly (94%), followed by (21%), which has a lower incidence; no was detected.
食源性病原体，尤其是耐药菌株，对全球公共卫生构成重大威胁。
探讨产超广谱β-内酰胺酶（ESBL）细菌在食物链中的出现和传播情况，重点关注湿市场中的肉鸡。
将标本接种到添加4μg/ml头孢噻肟的TBX培养基中，用于选择性分离产ESBL细菌。对分离株进行表型和基因型鉴定，包括使用针对该基因的特异性引物通过PCR进行菌种检测以及进行ESBL鉴定的双扩散协同试验。
共262/305（86%）份来自肉鸡和散养鸡的样本被确认为产ESBL细菌阳性。在肉鸡（91%）或散养鸡（100%）的产ESBL细菌中，主要鉴定出[具体细菌名称未给出]，其次分别为[另外两种细菌名称未给出]，占20%和23%。未鉴定出[特定基因名称未给出]基因。在50株产ESBL细菌中，[具体细菌名称未给出]（31株；肉鸡中占17.4%，散养鸡中占22.6%）被鉴定为同时携带[两种基因名称未给出]。
在受环境污染的食品中产ESBL细菌的流行率有所上升。应改进家禽养殖场的卫生和抗生素使用规定。鸡群中的ESBL基因主要为[具体基因名称未给出]（94%），其次为[另一基因名称未给出]（21%），其发生率较低；未检测到[特定基因名称未给出]。
