Negrelli João Gabriel Dumont, de Britto Rafael Maira Rafaela, Gazola Vitor Dib, Dos Santos Marcus Cesar Bochi Reis, Pilau Eduardo Jorge, Polli Andressa Domingos, Golias Halison Correia, de Almeida Tiago Tognolli, Polonio Julio Cesar
Laboratory of Microbial Biotechnology, Department of Biotechnology, Genetics and Cell Biology, Maringá State University, Maringá, Paraná, 87020-900, Brazil.
Department of Chemistry, Maringa State University, Maringá, Paraná, 87020-900, Brazil.
Int Microbiol. 2025 May 8. doi: 10.1007/s10123-025-00668-1.
Agricultural sustainability is vital to meet the growing global demand for food; therefore, the search for more sustainable options to replace traditional chemical products has gained attention due to their benefits. The sector has applied innovative microbial consortium approaches as a niche for exploring new bioproducts and metabolic pathways through microbial interactions. Thus, this study sought to select two endophytic bacterial strains with biocontrol activity to study their metabolic interactions in culture. For this, co-cultivation and axenic culture assays were carried out to evaluate the inhibition of Corynespora cassiicola, Sclerotinia sclerotiorum, Moniliophthora perniciosa, and Colletotrichum truncatum. After the production of antiphytopathogenic compound tests, two strains were selected: P. putida MG36 and B. amyloliquefaciens SS14. These bacteria were cultivated under three distinct conditions: axenic cultivation of SS14, axenic cultivation of MG36, and co-cultivation. The metabolites were extracted and analyzed by liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS). The results showed that both strains exhibited significant antifungal activity. B. amyloliquefaciens SS14 demonstrated 65% inhibition against C. truncatum, while P. putida MG36 showed 58% inhibition against S. sclerotiorum. Analysis of the chemical profiles revealed the presence of exclusive and shared metabolites, such as iturin A4 (antifungal lipopeptide) and macrolactin A (bioactive polyketide), under different culture conditions. In conclusion, P. putida MG36 and B. amyloliquefaciens SS14 show promise as biocontrol agents against phytopathogens, contributing to more sustainable agricultural practices.
农业可持续性对于满足全球日益增长的粮食需求至关重要；因此，由于其益处，寻求更可持续的选择来替代传统化学产品受到了关注。该领域已应用创新的微生物群落方法，作为通过微生物相互作用探索新生物产品和代谢途径的一个领域。因此，本研究旨在选择两种具有生物防治活性的内生细菌菌株，以研究它们在培养中的代谢相互作用。为此，进行了共培养和无菌培养试验，以评估对咖啡炭疽病菌、核盘菌、可可球二孢和烟草炭疽病菌的抑制作用。在进行抗植物病原菌化合物测试后，选择了两种菌株：恶臭假单胞菌MG36和解淀粉芽孢杆菌SS14。这些细菌在三种不同条件下培养：SS14的无菌培养、MG36的无菌培养和共培养。通过液相色谱-质谱联用（UHPLC-MS/MS）对代谢物进行提取和分析。结果表明，两种菌株均表现出显著的抗真菌活性。解淀粉芽孢杆菌SS14对烟草炭疽病菌的抑制率为65%，而恶臭假单胞菌MG36对核盘菌的抑制率为58%。化学图谱分析显示，在不同培养条件下存在独特和共享的代谢物，如伊枯草菌素A4（抗真菌脂肽）和大环内酯A（生物活性聚酮化合物）。总之，恶臭假单胞菌MG36和解淀粉芽孢杆菌SS14有望作为植物病原菌的生物防治剂，有助于实现更可持续的农业实践。
