Kashyap Nikhil, Singh Sandeep Kumar, Yadav Nisha, Singh Vipin Kumar, Kumari Madhuree, Kumar Dharmendra, Shukla Livleen, Bhardwaj Nikunj, Kumar Ajay
Department of Biotechnology, Noida International University, Greater Noida 203201, India.
Division of Microbiology, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi 110012, India.
Plants (Basel). 2023 Jun 28;12(13):2480. doi: 10.3390/plants12132480.
The considerable loss of crop productivity each year due to plant disease or pathogen invasion during pre- or post-harvest storage conditions is one of the most severe challenges to achieving the goals of food security for the rising global population. Although chemical pesticides severally affect the food quality and health of consumers, a large population relies on them for plant disease management. But currently, endophytes have been considered one of the most suitable biocontrol agents due to better colonization and acclimatization potential. However, a very limited number of endophytes have been used commercially as biocontrol agents. Isolation of endophytes and their screening to represent potential characteristics as biocontrol agents are considered challenging by different procedures. Through a web search using the keywords "endophytes as biocontrol agents" or "biocontrol mechanism of endophytes," we have succinctly summarised the isolation strategies and different in vitro and in vivo biocontrol screening methods of endophytic biocontrol agents in the present review. In this paper, biocontrol mechanisms of endophytes and their potential application in plant disease management have also been discussed. Furthermore, the registration and regulatory mechanism of the endophytic biocontrol agents are also covered.
在收获前或收获后的储存条件下，植物病害或病原体入侵每年都会导致作物生产力大幅损失，这是实现全球人口增长的粮食安全目标所面临的最严峻挑战之一。尽管化学农药会严重影响食品质量和消费者健康，但仍有大量人口依赖它们来防治植物病害。目前，由于内生菌具有更好的定殖和适应潜力，它们已被视为最适宜的生物防治剂之一。然而，仅有极少数内生菌被商业化用作生物防治剂。通过不同程序分离内生菌并筛选其作为生物防治剂的潜在特性颇具挑战性。通过使用关键词“作为生物防治剂的内生菌”或“内生菌的生物防治机制”进行网络搜索，我们在本综述中简要总结了内生菌生物防治剂的分离策略以及不同的体外和体内生物防治筛选方法。本文还讨论了内生菌的生物防治机制及其在植物病害管理中的潜在应用。此外，还涵盖了内生菌生物防治剂的注册和监管机制。
