Sun Wangyangji, Li Yu-An, Li Zewei, Li Guohui, Du Yuanzhao, Shi Huoying
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
Vet Microbiol. 2025 Jun;305:110546. doi: 10.1016/j.vetmic.2025.110546. Epub 2025 May 5.
In recent years, the advancement of nanomedicine has revolutionized the field of vaccine development. Numerous nanoparticle-based nano vaccine candidates have been continuously developed. Herein, we describe a universal avian influenza virus (AIV) vaccine candidate based on ferritin self-assembling nanoparticles (NPs). The ferritin fused with 3M2e was delivered by an recombinant Salmonella vector, resulting in the formation of a novel recombinant strain, rSC0130(pYA3342-3M2eNPs). The 3M2e-ferritin fusion protein was efficiently expressed and self-assembled into NPs within the recombinant strain. The delivery of the recombinant strain rSC0130(pYA3342-3M2eNPs), which carries the 3M2e-ferritin fusion NPs, induced robust specific cellular and humoral immune responses against AIV in vivo, ultimately providing chickens with effective cross-protection against challenges from H9N2 and H7N9 avian influenza viruses. Our study underscores the potential of the Salmonella platform for delivering protein-based NPs and offers new insights into the development of universal vaccines for AIVs.
近年来，纳米医学的进展彻底改变了疫苗开发领域。众多基于纳米颗粒的纳米疫苗候选物不断被研发出来。在此，我们描述了一种基于铁蛋白自组装纳米颗粒（NPs）的通用型禽流感病毒（AIV）疫苗候选物。与3M2e融合的铁蛋白由重组沙门氏菌载体递送，从而形成一种新型重组菌株rSC0130(pYA3342 - 3M2eNPs)。3M2e - 铁蛋白融合蛋白在重组菌株内高效表达并自组装成纳米颗粒。携带3M2e - 铁蛋白融合纳米颗粒的重组菌株rSC0130(pYA3342 - 3M2eNPs)的递送在体内诱导了针对AIV的强大特异性细胞免疫和体液免疫反应，最终为鸡提供了针对H9N2和H7N9禽流感病毒攻击的有效交叉保护。我们的研究强调了沙门氏菌平台递送基于蛋白质的纳米颗粒的潜力，并为AIV通用疫苗的开发提供了新的见解。
