Uy Neil Patrick, He Mei Tong, Lee Chang-Dae, Lee Yunji, Kang Ki Sung, Lee Sanghyun
Department of Plant Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea.
College of Korean Medicine, Gachon University, Seongnam, 13120, Republic of Korea.
J Ethnopharmacol. 2025 Jun 12;349:119933. doi: 10.1016/j.jep.2025.119933. Epub 2025 May 10.
Artemisia (Asteraceae) is a diverse genus of plants with ecological, economic, and therapeutic significance. Some species have been traditionally used for neuroprotection; however, their bioactive potential remains underexplored.
To identify Artemesia extracts and phytochemicals with neuroprotective effects that could provide alternative sources of functional foods, nutraceuticals, and therapeutics.
Twelve Artemisia extracts were tested for their neuroprotective effects, followed by phytochemical profiling and quantification using HPLC. Compounds with superior activity were subjected to western blot analysis.
Pretreatment with Artemesia stolonifera, A. rubripes, A. sieversiana, A. keiskeana, A. scoparia, and A. annua (125 μg/mL) attenuated glutamate-induced cytotoxicity. The findings revealed a significant (p < 0.05) heterogeneity in the phytochemical profiles of the species. Among the evaluated compounds, rutin (5) significantly protected HT22 cells from glutamate-induced damage. Pretreatment with rutin (5) upregulated Nrf2 and HO-1.
The study findings highlight the potential of Artemisia species as a source of bioactive chemicals in functional foods, nutraceuticals, and medicines. Further research on the bioactivity of these chemicals and the mechanisms responsible for their diversity could help develop cultivation strategies and potential medicinal applications.
蒿属（菊科）是一类具有生态、经济和治疗意义的多样植物。一些物种传统上被用于神经保护；然而，它们的生物活性潜力仍未得到充分探索。
鉴定具有神经保护作用的蒿属提取物和植物化学物质，可为功能性食品、营养保健品和治疗药物提供替代来源。
测试了12种蒿属提取物的神经保护作用，随后使用高效液相色谱法进行植物化学分析和定量。对活性较强的化合物进行蛋白质免疫印迹分析。
用匍匐委陵菜、红足蒿、大籽蒿、牡蒿、茵陈蒿和黄花蒿（125μg/mL）预处理可减轻谷氨酸诱导的细胞毒性。研究结果显示，这些物种的植物化学特征存在显著（p<0.05）异质性。在评估的化合物中，芦丁（5）显著保护HT22细胞免受谷氨酸诱导的损伤。用芦丁（5）预处理可上调Nrf2和HO-1。
研究结果突出了蒿属物种作为功能性食品、营养保健品和药物中生物活性化学物质来源的潜力。对这些化学物质的生物活性及其多样性产生机制的进一步研究，可能有助于制定种植策略和潜在的药用应用。
