Gang Gen, Gao Ruiheng, Li Ruizhen, Jin Xiao, Xing Yuanyuan, Yan Sumei, Xu Yuanqing, Shi Binlin
College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Antioxidants (Basel). 2025 Jul 18;14(7):885. doi: 10.3390/antiox14070885.
This study was conducted through in vivo and in vitro experiments and aimed to reveal the regulatory effect of water extract of L. (WEAA) on the antioxidant function of mutton sheep and the underlying mechanism. In the in vivo experiment, 32 Dorper × Han female sheep (3 months old; avg. body weight: 24 ± 0.09 kg) were allocated to four groups (eight lambs/group) and fed a diet containing 0, 500, 1000, and 1500 mg/kg WEAA, respectively. In the in vitro experiments, peripheral blood lymphocytes (PBLs) were cultured with different doses of WEAA (0, 25, 50, 100, 200, 400 µg/mL) to determine the optimal concentration, followed by a 2 × 2 factorial experiment with four treatment groups (six replicates per treatment group): the ML385(-)/WEAA(-) group, the ML385(-)/WEAA(+) group, the ML385(+)/WEAA(-) group, and the ML385(+)/WEAA(+) group. The results showed that WEAA supplementation dose-dependently increased serum, liver and spleen tissue total antioxidant capacity, glutathione peroxidase (GSH-Px), and catalase (CAT) activity while reducing malondialdehyde level ( < 0.05). Moreover, WEAA supplementation significantly upregulated the liver and spleen expression of , , , and ( < 0.05) while significantly downregulating the expression in a dose-dependent manner ( < 0.05), thereby activating the Keap1/Nrf2 pathway with the peak effect observed in the 1000 mg/kg WEAA group. Additionally, supplementation with 100 µg/mL of WEAA had significant antioxidation activity in the culture medium of PBLs. Its action mechanism involved the Keap1/Nrf2 pathway; specifically, WEAA exerted its antioxidant effect by upregulating the gene expression related to the Keap1/Nrf2 pathway. In conclusion, WEAA enhances sheep's antioxidant capacity by up-regulating Keap1/Nrf2 pathway genes and boosting antioxidant enzyme activity. The results provided experimental support for the potential application of WEAA in intensive mutton sheep farming.
本研究通过体内和体外实验进行，旨在揭示枸杞水提取物（WEAA）对肉羊抗氧化功能的调节作用及其潜在机制。在体内实验中，将32只杜泊×寒杂交母羊（3月龄；平均体重：24±0.09千克）分为四组（每组8只羔羊），分别饲喂含0、500、1000和1500毫克/千克WEAA的日粮。在体外实验中，用不同剂量的WEAA（0、25、50、100、200、400微克/毫升）培养外周血淋巴细胞（PBLs）以确定最佳浓度，随后进行2×2析因实验，设四个处理组（每组六个重复）：ML385（-）/WEAA（-）组、ML385（-）/WEAA（+）组、ML385（+）/WEAA（-）组和ML385（+）/WEAA（+）组。结果表明，补充WEAA可剂量依赖性地提高血清、肝脏和脾脏组织的总抗氧化能力、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性，同时降低丙二醛水平（P<0.05）。此外，补充WEAA可显著上调肝脏和脾脏中Nrf2、HO-1、GCLC、GCLM和Keap1的表达（P<0.05），同时以剂量依赖性方式显著下调Keap1的表达（P<0.05），从而激活Keap1/Nrf2通路，在1000毫克/千克WEAA组中观察到峰值效应。此外，在PBLs培养基中添加100微克/毫升的WEAA具有显著的抗氧化活性。其作用机制涉及Keap1/Nrf2通路；具体而言，WEAA通过上调与Keap1/Nrf2通路相关的基因表达发挥其抗氧化作用。总之，WEAA通过上调Keap1/Nrf2通路基因和提高抗氧化酶活性来增强绵羊的抗氧化能力。该结果为WEAA在肉羊集约化养殖中的潜在应用提供了实验支持。
