Liu He, Zhi Xinyi, Sun Jiameng, Li Yanru, Tao Li, Xiong Boyu, Lan Wenfei, Yu Lei, Song Shixin, Zhou Yuanhang
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China.
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China; National Engineering Research Center for Wheat and Corn Deep Processing, Jilin Agricultural University, Changchun 130118, China.
Int J Biol Macromol. 2025 Jun;311(Pt 3):144019. doi: 10.1016/j.ijbiomac.2025.144019. Epub 2025 May 6.
In this study, the structural characterization and neuroprotective effects of two Gastrodia elata Blume polysaccharides (GEP-1 and GEP-2) were investigated. GEP-1 and GEP-2, with molecular weights (Mws) of 3.698 kDa and 59.612 kDa, respectively, mainly comprised glucose. Methylation and nuclear magnetic resonance spectroscopic analyses indicated that the structure of GEP-1 was based on →4)-α-D-Glcp-(1 → and →4,6)-α-D-Glcp-(1→, with α-D-Glcp-(1 → and →6)-α-D-Glcp-(1 → as the side chains. The main chain of GEP-2 was →4)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1 → and →3,4)-α-D-Glcp-(1→, and α-D-Glcp-(1 → and →6)-α-D-Glcp-(1 → were the side chains. Both GEPs ameliorated copper exposure-induced HT-22 cell damage by inhibiting oxidative stress and inflammation. A comprehensive analysis revealed that the biological activity of GEP-2 was superior to that of GEP-1; this was related to its higher Mw, monosaccharide composition, type of glycosidic bonds, a greater degree of branching, and spatial conformation of GEP-2. These results provide new evidence for the neuroprotective effects of G. elata Blume polysaccharides against heavy metal exposure.
在本研究中，对两种天麻多糖（GEP - 1和GEP - 2）的结构特征和神经保护作用进行了研究。GEP - 1和GEP - 2的分子量（Mw）分别为3.698 kDa和59.612 kDa，主要由葡萄糖组成。甲基化和核磁共振光谱分析表明，GEP - 1的结构基于→4)-α-D-葡萄糖-(1→和→4,6)-α-D-葡萄糖-(1→，以α-D-葡萄糖-(1→和→6)-α-D-葡萄糖-(1→作为侧链。GEP - 2的主链为→4)-α-D-葡萄糖-(1→、→4,6)-α-D-葡萄糖-(1→和→3,4)-α-D-葡萄糖-(1→，α-D-葡萄糖-(1→和→6)-α-D-葡萄糖-(1→为侧链。两种天麻多糖均通过抑制氧化应激和炎症来改善铜暴露诱导的HT - 22细胞损伤。综合分析表明，GEP - 2的生物活性优于GEP - 1；这与其较高的分子量、单糖组成、糖苷键类型、更大的分支度以及GEP - 2的空间构象有关。这些结果为天麻多糖对重金属暴露的神经保护作用提供了新的证据。
