a Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology , Tianjin University of Science and Technology , Tianjin , P.R. China.
b Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture , Shanghai Ocean University , Shanghai , P.R. China.
Nat Prod Res. 2019 Jun;33(12):1721-1726. doi: 10.1080/14786419.2018.1434643. Epub 2018 Feb 2.
The structural properties and Angiotensin-I converting enzyme (ACE) inhibition activities of a polysaccharide (PGE) extracted from Blume were investigated. PGE was extracted using hot water and purified by Sephadex G-200 followed by ultra-filtration. The structural characterisation of PGE was analysed by FT-IR, NMR spectroscopy, specific rotation determination, periodate oxidation-smith degradation, methylation analysis, GC-MS and Congo red test. The results revealed that PGE was composed by glucose, with an average molecular weight of 1.54 × 10 kDa. The structure of PGE was 1→3 and 1→4,6-branched-glucopyranose that had a linear backbone of (1 → 4)-linked-d-glucopyranose (Glcp). ACE-inhibitory activity results showed that PGE was efficient to inhibit ACE and the IC value was 0.66 mg/mL.
从 Blume 中提取的多糖 (PGE) 的结构特性和血管紧张素转化酶 (ACE) 抑制活性进行了研究。PGE 采用热水提取，然后通过 Sephadex G-200 进一步纯化，最后通过超滤。通过傅里叶变换红外光谱 (FT-IR)、核磁共振波谱 (NMR)、比旋光度测定、高碘酸盐氧化-Smith 降解、甲基化分析、GC-MS 和刚果红试验分析了 PGE 的结构特征。结果表明，PGE 由葡萄糖组成，平均分子量为 1.54×10 kDa。PGE 的结构为 1→3 和 1→4、6-支链葡萄糖吡喃糖，具有 (1 → 4)-连接的-d-葡萄糖吡喃糖 (Glcp) 的线性主链。ACE 抑制活性结果表明，PGE 对 ACE 有抑制作用，IC 值为 0.66mg/mL。
