儿童高血压相关的肠道微生物群改变。

Alteration of gut microbiota associated with hypertension in children.

作者信息

Sun Jiahong, Yang Liu, Ma Chuanwei, Yang Lili, Zhao Min, Magnussen Costan G, Xi Bo

机构信息

Department of Preventive Medicine, School of Public Health, Guangdong Medical University, Dongguan, Guangdong, China.

Department of Epidemiology, School of Public Health, Cheeloo College of Medicine, Shandong University, 44 Wen Hua Xi Road, Jinan, 250012, Shandong, China.

出版信息

BMC Microbiol. 2025 May 8;25(1):282. doi: 10.1186/s12866-025-03999-1.

DOI:10.1186/s12866-025-03999-1

PMID:40340772

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12060425/

Abstract

BACKGROUND

The association of disturbance in gut microbiota with hypertension (HTN) defined on three separate occasions among children and adolescents remains unclear. In this study, we aimed to compare the differences in gut microbiota composition and diversity between children with HTN and those with normal blood pressure (BP).

METHODS

Data and stool samples were collected from the second follow-up of a childhood cardiovascular health cohort study in 2021. 16 S ribosomal RNA gene sequencing was conducted to determine the relative abundance of microbial taxa in 51 children aged 10-14 years with HTN and 51 children with normal BP.

RESULTS

Compared with children with normal BP, those with HTN had decreased gut microbiome diversity. At the genus level, after adjusting for the false discovery rate (FDR), the proportions of several gut microbiota such as Blautia (P=0.042), Coprococcus (P=0.042), Eubacterium_ventriosum_group (P=0.027), Christensenellaceae_R-7_group (P=0.027), and norank_f__Lachnospiraceae (P=0.015) significantly decreased in children with HTN compared to those with normal BP. Receiver operating characteristic analysis, net reclassification improvement (NRI) and integrated discrimination improvement (IDI) were performed and showed that the genera norank_f__Lachnospiraceae and Dorea significantly enhanced the ability of body mass index to differentiate between children with HTN and those with normal BP (area under the receiver operating characteristic curve: 0.95, 95% confidence interval 0.91-0.99; NRI > 0; IDI = 0.12, P < 0.05). Phylogenetic Investigation of Communities by Reconstruction of Unobserved States showed that the mean proportions of cofactors and vitamins metabolism pathway and the glycan anabolism pathway were higher in children with HTN.

CONCLUSIONS

Disturbances in the abundance and diversity of gut microbiota may contribute to the development of HTN in children. Gut microbiota biomarkers may be of significant importance in the early identification and diagnosis of childhood HTN.

CLINICAL TRIAL NUMBER

Not applicable.

摘要

背景

肠道微生物群紊乱与儿童和青少年在三个不同时间点定义的高血压（HTN）之间的关联仍不清楚。在本研究中，我们旨在比较高血压儿童和血压正常（BP）儿童肠道微生物群组成和多样性的差异。

方法

数据和粪便样本收集自2021年一项儿童心血管健康队列研究的第二次随访。对51名10 - 14岁高血压儿童和51名血压正常儿童进行16S核糖体RNA基因测序，以确定微生物类群的相对丰度。

结果

与血压正常儿童相比，高血压儿童的肠道微生物群多样性降低。在属水平上，校正错误发现率（FDR）后，高血压儿童中一些肠道微生物群的比例，如布劳特氏菌属（P = 0.042）、粪球菌属（P = 0.042）、真杆菌属-腹菌菌群（P = 0.027）、克里斯滕森菌科-R-7菌群（P = 0.027）和无分类阶元_f__毛螺菌科（P = 0.015），与血压正常儿童相比显著降低。进行了受试者工作特征分析、净重新分类改善（NRI）和综合判别改善（IDI），结果显示无分类阶元_f__毛螺菌科和多雷亚菌属显著增强了体重指数区分高血压儿童和血压正常儿童的能力（受试者工作特征曲线下面积：0.95，95%置信区间0.91 - 0.99；NRI > 0；IDI = 0.12，P < 0.05）。通过重建未观察状态对群落进行系统发育研究表明，高血压儿童中辅因子和维生素代谢途径以及聚糖合成代谢途径的平均比例较高。