Shirazpour Sara, Taheri Farahnaz, Sepehri Gholamreza, Zangiabadizadeh Mahla, Zangiabadi Mostafa, Hosseini Najmeh Sadat, Sheikhi Sara, Raeini Azadeh Shahrokhi, Tezerji Sara Sheibani
Institute of Neuropharmacology, Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Neurology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Brain Behav. 2025 May;15(5):e70539. doi: 10.1002/brb3.70539.
The current study aimed to investigate the impacts of 8-week high-intensity interval training (HIIT) and Ritalin (RIT), alone and in combination, on cognitive functions and hippocampal oxidative parameters following chronic ethanol consumption in male rats.
A total of 56 adult male rats were divided into 8 groups and received one of the following treatments: ethanol 20% (ET) (3 mL/kg/day, orally, 5 consecutive days/week in weeks 1-4, and 3 consecutive days/week in weeks 4-8), RIT (10 mg/kg, intraperitoneally, three consecutive times/week for 8 weeks), HIIT + SAL (five consecutive times/week for 8 weeks + saline injection), or saline (1 mL/day, intraperitoneally, three consecutive times/week for 8 weeks). Cognitive performance was assessed using the Morris water maze (MWM) and passive avoidance tasks. Oxidative stress markers, including malondialdehyde (MDA), glutathione peroxidase (GPx), and total antioxidant capacity (TAC), were measured in the hippocampus using thiobarbituric acid reactive substances (TBARS) and ferric reduction antioxidant power (FRAP). Nitric oxide (NO) level in the hippocampus was determined using an NO Assay Kit (Natrix, Arman Biotech, Iran).
Chronic ethanol administration impaired cognitive functions. However, RIT, HIIT, and their combination significantly improved these impairments. Furthermore, RIT increased ethanol-induced oxidative stress, whereas HIIT reduced it, even in the combination group.
Chronic ethanol consumption caused learning and memory deficits and disrupted oxidant/antioxidant balance in the hippocampus of rats. HIIT potentially improved memory impairments by restoring this balance, whereas RIT ameliorated cognitive dysfunction through a mechanism that requires further investigation.
本研究旨在调查8周高强度间歇训练（HIIT）和利他林（RIT）单独及联合使用，对慢性乙醇摄入雄性大鼠认知功能和海马氧化参数的影响。
总共56只成年雄性大鼠被分为8组，接受以下治疗之一：20%乙醇（ET）（3毫升/千克/天，口服，第1 - 4周每周连续5天，第4 - 8周每周连续3天），RIT（10毫克/千克，腹腔注射，每周连续3次，共8周），HIIT + 生理盐水（每周连续5次，共8周 + 生理盐水注射），或生理盐水（1毫升/天，腹腔注射，每周连续3次，共8周）。使用莫里斯水迷宫（MWM）和被动回避任务评估认知表现。使用硫代巴比妥酸反应物质（TBARS）和铁还原抗氧化能力（FRAP）测量海马中的氧化应激标志物，包括丙二醛（MDA）、谷胱甘肽过氧化物酶（GPx）和总抗氧化能力（TAC）。使用一氧化氮检测试剂盒（Natrix，Arman Biotech，伊朗）测定海马中的一氧化氮（NO）水平。
慢性乙醇给药损害了认知功能。然而，RIT、HIIT及其联合使用显著改善了这些损害。此外，RIT增加了乙醇诱导的氧化应激，而HIIT降低了氧化应激，即使在联合组中也是如此。
慢性乙醇摄入导致大鼠学习和记忆缺陷，并破坏了海马中的氧化剂/抗氧化剂平衡。HIIT可能通过恢复这种平衡来改善记忆损害，而RIT通过一种需要进一步研究的机制改善认知功能障碍。
