Bejeshk Mohammad Abbas, Beik Ahmad, Aminizadeh Amir Hashem, Salimi Fouzieh, Bagheri Fatemeh, Sahebazzamani Maryam, Najafipour Hamid, Rajizadeh Mohammad Amin
Student Research Committee, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Naunyn Schmiedebergs Arch Pharmacol. 2023 Jun;396(6):1235-1245. doi: 10.1007/s00210-023-02398-5. Epub 2023 Jan 28.
Allergic asthma is an inflammatory and chronic condition, which is the most common asthma phenotype. It is usually defined by sensitivity to environmental allergens and leads to the narrowing of the airways. Around 300 million individuals are suffering from asthma worldwide. The purpose of the current research was to evaluate the effect of perillyl alcohol (PA) on oxidative stress and inflammation parameters in rats with allergic asthma. Experimental asthma was induced by ovalbumin (OVA) sensitization and inhalation in five groups of rats including control, asthma, asthma + vehicle, asthma + PA, and asthma + dexamethasone (Dexa). PA (50 mg/kg) or Dexa (2.5 mg/kg) were administered intraperitoneally for seven consecutive days following asthma induction. Histopathological evaluation was performed via hematoxylin and eosin (H&E) and Masson's trichrome staining. The enzyme-linked immunosorbent assay (ELISA) was used for the evaluation of the cytokine levels, including tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, IL-17, and IL-10, as well as oxidative stress biomarkers including reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant capacity (TAC), and glutathione peroxidase (GPx) in the lung tissue and bronchoalveolar lavage fluid (BALF). Real-time polymerase chain reaction (PCR) was utilized for assessing the mRNA expression of FOXP3 and GATA3 and western blot analysis was used for the measurement of nuclear factor kappa B (NF-κB) protein expression. PA and Dexa decreased the pathological alterations and the expression levels of inflammatory factors (cytokines, GATA3, and NF-κB) in the lung tissue and BALF of asthmatic rats. PA restored GPx, SOD, and TAC levels and reduced ROS, MDA, nitrite, and total protein in the lung and BALF. Overall, our findings demonstrated that PA can be used as a therapeutic agent in asthma patients, but it is essential to monitor its effects in future clinical studies.
过敏性哮喘是一种炎症性慢性疾病，是最常见的哮喘表型。它通常由对环境过敏原的敏感性定义，并导致气道狭窄。全球约有3亿人患有哮喘。当前研究的目的是评估紫苏醇（PA）对过敏性哮喘大鼠氧化应激和炎症参数的影响。通过卵清蛋白（OVA）致敏和吸入在五组大鼠中诱导实验性哮喘，包括对照组、哮喘组、哮喘+赋形剂组、哮喘+PA组和哮喘+地塞米松（Dexa）组。在哮喘诱导后连续7天腹腔注射PA（50mg/kg）或Dexa（2.5mg/kg）。通过苏木精和伊红（H&E）染色以及Masson三色染色进行组织病理学评估。酶联免疫吸附测定（ELISA）用于评估细胞因子水平，包括肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-6、IL-17和IL-10，以及氧化应激生物标志物，包括肺组织和支气管肺泡灌洗液（BALF）中的活性氧（ROS）、超氧化物歧化酶（SOD）、丙二醛（MDA）、总抗氧化能力（TAC）和谷胱甘肽过氧化物酶（GPx）。实时聚合酶链反应（PCR）用于评估FOXP3和GATA3的mRNA表达，蛋白质印迹分析用于测量核因子κB（NF-κB）蛋白表达。PA和Dexa减少了哮喘大鼠肺组织和BALF中的病理改变以及炎症因子（细胞因子、GATA3和NF-κB）的表达水平。PA恢复了肺和BALF中GPx、SOD和TAC水平，并降低了ROS、MDA、亚硝酸盐和总蛋白。总体而言，我们的研究结果表明PA可作为哮喘患者的治疗药物，但在未来的临床研究中监测其效果至关重要。
