Yaqubi Ahmad Khalil, Astuti Suryani Dyah, Zaidan Andi Hamim, Qadir Karwan Wasman, Razak Nasrul Anuar Abd, Permatasari Perwira Annissa Dyah, Nurdin Dezy Zahrotul Istiqomah
Department of Physics, Faculty of Science and Technology, Airlangga University, Surabaya, 60115, Indonesia.
Department of Physics, College of Education, Salahaddin University-Erbil, 44002 Erbil, Kurdistan Region, Iraq.
Vet World. 2025 Mar;18(3):547-557. doi: 10.14202/vetworld.2025.547-557. Epub 2025 Mar 9.
Wound healing is a complex biological process often hindered by bacterial infections, particularly , including methicillin-resistant (MRSA). Conventional antibiotic treatments face challenges due to antimicrobial resistance, necessitating alternative approaches. This study evaluates the efficacy of blue laser-activated silver nanoparticles synthesized from grape seed extract (GSE-AgNPs) in promoting wound healing and reducing bacterial load in Wistar mice.
GSE-AgNPs were synthesized and characterized before application. Wistar mice were divided into three experimental groups: (1) blue laser therapy alone, (2) GSE-AgNPs alone, and (3) combined treatment. A 2.5 cm incision was created on the dorsal side of each mouse, and treatments were administered on days 1, 3, and 5 post-incision. Wound healing progression was assessed through histopathology, bacterial colony counts, and immune response markers (lymphocyte and monocyte levels). Statistical analysis was performed using two-way analysis of variance, followed by Tukey's test.
Compared with individual treatments, the combination of GSE-AgNPs and blue laser therapy significantly improved wound healing outcomes. The combined therapy led to a 60% reduction in wound size and an 88.73% decrease in bacterial load. Immune response markers showed enhanced activity, with lymphocyte levels increasing by 75% and monocyte levels rising by 50%, indicating a stronger immune response. Histopathological analysis confirmed accelerated re-epithelialization and increased fibroblast activity in the combination therapy group.
The findings suggest that blue laser-activated GSE-AgNPs provide a promising alternative for enhancing wound healing and bacterial infection control, particularly against MRSA. The synergistic effect of nanoparticles and laser activation promotes immune modulation and tissue regeneration. Future research should explore clinical applications and dosage optimization for human use.
伤口愈合是一个复杂的生物学过程，常受到细菌感染的阻碍，尤其是耐甲氧西林金黄色葡萄球菌（MRSA）。由于抗菌耐药性，传统抗生素治疗面临挑战，因此需要替代方法。本研究评估了由葡萄籽提取物合成的蓝色激光激活银纳米颗粒（GSE-AgNPs）在促进Wistar小鼠伤口愈合和减少细菌载量方面的疗效。
在应用前对GSE-AgNPs进行合成和表征。将Wistar小鼠分为三个实验组：（1）单独蓝色激光治疗组，（2）单独GSE-AgNPs治疗组，（3）联合治疗组。在每只小鼠的背部制作一个2.5厘米的切口，并在切口后的第1、3和5天进行治疗。通过组织病理学、细菌菌落计数和免疫反应标志物（淋巴细胞和单核细胞水平）评估伤口愈合进程。使用双向方差分析进行统计分析，随后进行Tukey检验。
与单独治疗相比，GSE-AgNPs与蓝色激光治疗的联合显著改善了伤口愈合结果。联合治疗使伤口大小减少了60%，MRSA细菌载量减少了88.73%。免疫反应标志物显示活性增强，淋巴细胞水平增加了75%，单核细胞水平上升了50%，表明免疫反应更强。组织病理学分析证实联合治疗组的再上皮化加速和成纤维细胞活性增加。
研究结果表明，蓝色激光激活的GSE-AgNPs为增强伤口愈合和控制细菌感染，特别是针对MRSA，提供了一种有前景的替代方法。纳米颗粒与激光激活的协同作用促进了免疫调节和组织再生。未来的研究应探索其在人类中的临床应用和剂量优化。
