Mohammed Hamdoon A, Amin Mohammed A, Zayed Gamal, Hassan Yassar, El-Mokhtar Mohamed, Saddik Mohammed S
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah, 51452, Kingdom of Saudi Arabia.
Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University, Cairo, 11884, Egypt.
J Antibiot (Tokyo). 2023 May;76(5):291-300. doi: 10.1038/s41429-023-00603-4. Epub 2023 Feb 28.
The multi-drug resistant Staph. aureus strain, Methicillin-resistant Staphylococcus aureus (MRSA), is an emerging pathogen that could penetrate skin cuts and wounds, causing a life-threatening condition. The green biosynthesis of silver nanoparticles with liquorice extract has been demonstrated over several years for anticancer and antioxidant effects, as well as antibacterial effect against both Gram-positive and Gram-negative bacteria. The study was designed to evaluate the synergistic in vivo and in vitro wound healing and anti-MRSA activity of decorated liquorice silver nanoparticles (LD-AgNPs). The LD-AgNPs were prepared by thoroughly mixing diluted liquorice extract with AgNO at room temperature. The prepared nanoparticles were characterized by size measurement, IR spectroscopy, TEM imaging, and X-ray diffraction. The in vitro and in vivo antibacterial and wound healing testing were also performed. The obtained LD-AgNPs were spherical in shape and had a hydrodynamic size of about 50.16 ± 5.37 nm. Moreover, they showed potent antibacterial activity against Gram-positive and Gram-negative resistant bacteria, produced a significantly higher level of procollagen type I compared to either liquorice extract or standard silver sulfadiazine, and promoted the wound healing process in rabbits. The formulation of silver nanoparticles with liquorice extract showed synergetic effects in enhancing the treatment of wounds, with significant antibacterial activity against E. coli and MRSA.
多重耐药金黄色葡萄球菌菌株，即耐甲氧西林金黄色葡萄球菌（MRSA），是一种新兴病原体，可穿透皮肤伤口，引发危及生命的状况。数年来，用甘草提取物进行银纳米颗粒的绿色生物合成已被证明具有抗癌、抗氧化作用，以及对革兰氏阳性菌和革兰氏阴性菌的抗菌作用。本研究旨在评估修饰的甘草银纳米颗粒（LD-AgNPs）在体内和体外协同促进伤口愈合及抗MRSA的活性。通过在室温下将稀释的甘草提取物与硝酸银充分混合来制备LD-AgNPs。通过尺寸测量、红外光谱、透射电镜成像和X射线衍射对制备的纳米颗粒进行表征。还进行了体外和体内抗菌及伤口愈合测试。所获得的LD-AgNPs呈球形，流体动力学尺寸约为50.16±5.37nm。此外，它们对革兰氏阳性和革兰氏阴性耐药菌表现出强大的抗菌活性，与甘草提取物或标准磺胺嘧啶银相比，产生的I型前胶原水平显著更高，并促进了兔子的伤口愈合过程。用甘草提取物制备银纳米颗粒在增强伤口治疗方面显示出协同作用，对大肠杆菌和MRSA具有显著的抗菌活性。
