Lundgaard Maja Hjelm, Bruun Niels Henrik, Handberg Aase, Andersen Stig, Andersen Stine Linding
Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark.
Department of Clinical Medicine, Aalborg University, Aalborg, Denmark.
J Appl Lab Med. 2025 Jul 1;10(4):949-962. doi: 10.1093/jalm/jfaf064.
Placental dysfunction is important to recognize, and more evidence is needed on the dynamics of the placental biomarkers, soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF), in early pregnancy. This study aimed to establish reference intervals for placental biomarkers in early pregnancy.
This was a retrospective cohort study of pregnant women (n = 702) in the North Denmark Region, 2013, who had blood samples drawn in early pregnancy including measurements of sFlt-1 and PlGF (Kryptor Compact, Thermofisher Scientific). Reference intervals were established, and the association between sFlt-1 and PlGF and maternal characteristics (age, body mass index [BMI], country of birth, and smoking in pregnancy) was evaluated using linear regression analyses and reported as adjusted beta coefficient (aβ) with 95% confidence intervals (CIs).
The placental biomarkers showed a dynamic trend with higher levels for increasing week of pregnancy; however, sFlt-1 reached a plateau around week 10 of pregnancy. Higher maternal age associated with higher sFlt-1 and PlGF (sFlt-1: aβ 1.02 [95% CI, 1.01-1.03], PlGF: aβ 1.02 [95% CI, 1.01-1.03]), and higher maternal BMI associated with lower sFlt-1 (aβ 0.97 [95% CI, 0.96-0.98]). Furthermore, the level of PlGF was higher among women born outside of Denmark (aβ 1.17 [95% CI, 1.03-1.34]) compared to women born in Denmark and among smokers (aβ 1.56 [95% CI, 1.38-1.78]) compared to nonsmokers.
In a large cohort of pregnant women in Denmark, levels of sFlt-1 and PlGF increased during early pregnancy and were influenced by a series of maternal characteristics.
胎盘功能障碍的识别很重要，关于妊娠早期胎盘生物标志物可溶性fms样酪氨酸激酶-1（sFlt-1）和胎盘生长因子（PlGF）动态变化的证据仍需更多。本研究旨在确定妊娠早期胎盘生物标志物的参考区间。
这是一项对2013年丹麦北部地区702名孕妇进行的回顾性队列研究，这些孕妇在妊娠早期采集了血样，包括sFlt-1和PlGF的检测（Kryptor Compact，赛默飞世尔科技公司）。确定了参考区间，并使用线性回归分析评估sFlt-1和PlGF与母亲特征（年龄、体重指数[BMI]、出生国家和孕期吸烟情况）之间的关联，并报告为调整后的β系数（aβ）及95%置信区间（CIs）。
胎盘生物标志物呈现动态变化趋势，随着孕周增加水平升高；然而，sFlt-1在妊娠第10周左右达到平台期。母亲年龄越大，sFlt-1和PlGF水平越高（sFlt-1：aβ 1.02 [95% CI，1.01 - 1.03]，PlGF：aβ 1.02 [95% CI，1.01 - 1.03]），母亲BMI越高，sFlt-1水平越低（aβ 0.97 [95% CI，0.96 - 0.98]）。此外，与丹麦出生的女性相比，丹麦境外出生的女性PlGF水平更高（aβ 1.17 [95% CI，1.03 - 1.34]），与非吸烟者相比，吸烟者的PlGF水平更高（aβ 1.56 [95% CI，1.38 - 1.78]）。
在丹麦的一大群孕妇中，sFlt-1和PlGF水平在妊娠早期升高，并受到一系列母亲特征的影响。
