Yin Jiajia, Sun Wenyu, Xiong Hongjie, Yao Wenyan, Liu Xiaohui, Jiang Hui, Wang Xuemei
State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
Biomaterials. 2025 Nov;322:123388. doi: 10.1016/j.biomaterials.2025.123388. Epub 2025 May 6.
Based on the unique biological advantages of bacteria and their derivatives, biosynthetic nanomaterials have been widely used in the field of tumor therapy. Although conventional bacterial treatments demonstrate potential in activating tumor immunity, their efficacy in inhibiting tumor growth remains constrained. In this study, a photoactivated hydrogen sulfide (HS) generator was successfully prepared by in-situ engineering of bacteria, after Pt/MoS nanocomposites were in-situ generated by Escherichia coli (E. coli) and loaded with photosensitizer Ce6. This engineered-bacteria has been proved to have good tumor targeting ability and can enhance the effect of photodynamic therapy in the hypoxic tumor microenvironment. While reactive oxygen species (ROS) is effectively released, the fragmentation of bacteria can accelerate the release of abundant HS, and promote tumor-specific HS gas therapy, which can effectively remodel the tumor microenvironment and promote the activation of anti-tumor immunotherapy. This engineered bacteria not only improves the tumor specificity and effectiveness of HS treatment, but also provides a new idea for nanomaterials in bacterial-mediated synergistic cancer treatment.
基于细菌及其衍生物独特的生物学优势，生物合成纳米材料已在肿瘤治疗领域得到广泛应用。尽管传统的细菌治疗在激活肿瘤免疫方面显示出潜力，但其在抑制肿瘤生长方面的疗效仍然有限。在本研究中，通过细菌原位工程成功制备了一种光活化硫化氢（HS）发生器，此前大肠杆菌（E. coli）原位生成了Pt/MoS纳米复合材料并负载了光敏剂Ce6。这种工程菌已被证明具有良好的肿瘤靶向能力，并且可以在缺氧的肿瘤微环境中增强光动力治疗的效果。在有效释放活性氧（ROS）的同时，细菌的裂解可以加速大量HS的释放，并促进肿瘤特异性HS气体治疗，从而有效重塑肿瘤微环境并促进抗肿瘤免疫治疗的激活。这种工程菌不仅提高了HS治疗的肿瘤特异性和有效性，还为纳米材料在细菌介导的协同癌症治疗中提供了新思路。
