Establishment of Agrobacterium-mediated genetic transformation and CRISPR/Cas9-guided gene editing in Elymus nutans.
作者信息
Li Cheng, Peng Xiaomei, Zhang Zhengshe, Liu Yaling, Pedro García-Caparrós, Fu Chunxiang, Yang Yongping, Dong Quanmin, Duan Yuanwen, Sun Xudong
机构信息
Yunnan Key Laboratory of Crop Wild Relatives Omics, The Germplasm Bank of Wild Species, Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, 810016, Qinghai, China.
出版信息
J Plant Physiol. 2025 Jul;310:154513. doi: 10.1016/j.jplph.2025.154513. Epub 2025 May 5.
Elymus nutans, an allohexaploid (2n = 6x = 42) species with a StStHHYY genome, is a native perennial in the alpine grasslands of the Qinghai-Xizang Plateau, and has been widely used for artificial pasture and ecological restoration as a forage grass with highest yield on the plateau. Nevertheless, the lack of a stable transformation system has impeded further efforts to trait improvement of E. nutans. In the present study, we established a reliable Agrobacterium-mediated genetic transformation system for E. nutans, and successfully generated EnTCP4-edited plants using the CRISPR/Cas9 system. The editing efficiency achieved 19.23 % in E. nutans. Knocking out EnTCP4 significantly delayed flowering and enhanced water-deficit stress resistance. This research represents a significant breakthrough in the genetic transformation and gene editing of E. nutans, laying a technological foundation to gain insight into gene functions and molecular breeding in E. nutans.
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