National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College Pakistan Institute of Engineering and Applied Sciences (PIEAS), Jhang Road, Faisalabad, Pakistan.
Department of Biotechnology, Shahid Benazir Bhatoo University Sheringal, Upper Dir, Khyber Pakhtunkhwa, Pakistan.
Mol Biotechnol. 2024 Aug;66(8):1836-1852. doi: 10.1007/s12033-023-00826-8. Epub 2023 Aug 13.
Plant transformation based on Agrobacterium-mediated transformation is a technique that mimics the natural agrobacterium system for gene(s) introduction into crops. Through this technique, various crop species have been improved/modified for different trait/s, showing a successful genetic transformation so far. This technique has many advantages over other transformation methods such as stable integration of transgene, cost effective. However, there are many limitations of this technology such as mostly the crops are recalcitrant to agrobacterium, low transformation efficiency, transgene integration as well as off targets. So, it's very important to explore the major limitations and possible solutions for Agrobacterium-mediated transformation in order to increase its genetic transformation efficiency. Therefore, the present review article gives a comprehensive study how the transgenic crops are developed using Agrobacterium-mediated transformation, crops that have already been modified through this method, and risks associated with transgenic plants based on Agrobacterium-mediated transformation. Moreover, the challenges and problems associated with Agrobacterium-mediated transformation and how those problems can be solved in future for a successful genetic transformation of crops using modern biotechnology techniques such as CRISPR/Cas9 systems. The present review article will be really helpful for the audience those working on Genome editing of crops using Agrobacterium-mediated transformation and will opens many ways for future plant genetic transformation.
基于农杆菌介导转化的植物转化是一种模拟天然农杆菌系统将基因（s）导入作物的技术。通过该技术，各种作物已经被改良/修饰用于不同的特性/，迄今为止已显示出成功的遗传转化。与其他转化方法相比，该技术具有许多优点，例如转基因的稳定整合、成本效益高。然而，该技术也存在许多局限性，例如大多数作物对农杆菌具有抗性、转化效率低、转基因整合以及脱靶。因此，探索农杆菌介导转化的主要限制和可能的解决方案对于提高其遗传转化效率非常重要。因此，本文综述了如何利用农杆菌介导转化来开发转基因作物、已经通过该方法修饰的作物以及基于农杆菌介导转化的转基因植物的风险。此外，还讨论了农杆菌介导转化相关的挑战和问题，以及如何在未来利用现代生物技术技术（如 CRISPR/Cas9 系统）解决这些问题，以实现作物的成功遗传转化。本文综述将对从事利用农杆菌介导转化进行作物基因组编辑的研究人员非常有帮助，并为未来的植物遗传转化开辟了许多途径。
