Yuan Yang, Wang Xiaoyan, Yan Xiaodi, He Nannan, Lu Xiaojian, Yang Jingyu, Xie Xinwei, Yuan Huiyao, Chen Naixin, Liu Yinbo, Ren Hongan, Zhang Runzhao, Cui Lina, Ren Pengcheng, Lin Sirui, Cheng Shuhan, Yang Xiaolong, Guo Yifei, Li Rong, Yan Tianyi, Guo Jingtao, Xiao Zhenyu, Wei Yulei, Yu Leqian
State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Cell Stem Cell. 2025 Jun 5;32(6):1006-1024.e5. doi: 10.1016/j.stem.2025.04.007. Epub 2025 May 8.
The Carnegie stage 9 (CS9) embryo is a pivotal phase signifying the conclusion of gastrulation and the onset of early organogenesis, crucial for initiating major organ system development. Utilizing spatial transcriptomics, we analyzed an intact CS9 human embryo in a spatially detailed manner. Through the examination of 75 transverse cryosections, we digitally reconstructed a 3D model, allowing us to identify diverse cell types, including those from brain and spine regions, the primitive gut tube, distinct somite formation stages, somatic mesoderm, splanchnic mesoderm, etc. Notably, we observed two distinct trajectories of hindbrain development, pinpointed the isthmic organizer at the midbrain-hindbrain boundary, delineated the bi-layered structure of neuromesodermal progenitor (NMP) cells, and described the early aorta formation and primordial germ cells (PGCs) presence in the aorta-gonad-mesonephros (AGM) region. This study provides key insights into the transcriptomic and spatial intricacies shaping the human body plan.
卡内基第9阶段（CS9）胚胎是一个关键阶段，标志着原肠胚形成的结束和早期器官发生的开始，这对于启动主要器官系统的发育至关重要。利用空间转录组学，我们以空间详细的方式分析了一个完整的CS9人类胚胎。通过检查75个横向冷冻切片，我们数字重建了一个三维模型，使我们能够识别不同的细胞类型，包括来自脑和脊柱区域、原始肠管、不同体节形成阶段、体壁中胚层、脏壁中胚层等的细胞类型。值得注意的是，我们观察到后脑发育的两条不同轨迹，确定了中脑-后脑边界处的峡部组织者，描绘了神经中胚层祖细胞（NMP）的双层结构，并描述了早期主动脉形成以及主动脉-性腺-中肾（AGM）区域中原始生殖细胞（PGC）的存在。这项研究为塑造人体蓝图的转录组学和空间复杂性提供了关键见解。
