Francis Thomas, Soendenbroe Casper, Lazarus Norman R, Mackey Abigail L, Harridge Stephen D R
Centre for Human & Applied Physiological Sciences, Basic & Medical Biosciences, Faculty of Life Science & Medicine, King's College London, London, UK.
Institute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery, Copenhagen University Hospital-Bispebjerg and Frederiksberg, Copenhagen, Denmark.
J Muscle Res Cell Motil. 2025 May 10. doi: 10.1007/s10974-025-09696-w.
This review arises from the symposium held in honour of Prof Jenny Morgan at UCL in 2024 and the authors would like to acknowledge the outstanding contribution that Prof Morgan has made to the field of translational muscle cell biology. Prof Morgan published a review article in 2010 entitled: Are human and mice satellite cells really the same? In which the authors highlighted differences between species which are still pertinent to skeletal muscle cell culture studies today. To our knowledge there are no comprehensive reviews which outline the considerable work that has been undertaken using human primary skeletal muscle origin cells as the main model system. This review highlights the multitude of muscle biology that has been investigated using human primary cells, as well as discussing the advantages and disadvantages over other cell models. We also discuss future directions for primary cell culture models utilising the latest technologies in cell type specificity and culture systems.
本综述源于2024年在伦敦大学学院为纪念珍妮·摩根教授而举办的研讨会，作者们在此感谢摩根教授对转化性肌肉细胞生物学领域所做出的杰出贡献。摩根教授在2010年发表了一篇综述文章，题为《人类和小鼠的卫星细胞真的一样吗？》，文中作者强调了物种间的差异，这些差异在当今骨骼肌细胞培养研究中仍然具有相关性。据我们所知，目前尚无全面的综述概述以人类原代骨骼肌来源细胞作为主要模型系统所开展的大量工作。本综述重点介绍了利用人类原代细胞所开展的众多肌肉生物学研究，同时也讨论了与其他细胞模型相比的优缺点。我们还利用细胞类型特异性和培养系统方面的最新技术，探讨了原代细胞培养模型的未来发展方向。
