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环状核糖核酸RPPH1通过招募并结合真核翻译起始因子4A3促进膀胱尿路上皮癌增殖和上皮-间质转化。

Circ_RPPH1 promotes bladder urothelium carcinoma proliferation and EMT by recruiting and binding to EIF4 A3.

作者信息

Liu HuaWei, Ma JunMin, Yan Xia

机构信息

Department of Urology, Deyang People's Hospital, Sichuan Province, Deyang City, 618000, China.

Department of Urology, Huaian Hospital of Huaian City, Huaian City, Jiangsu Province, 223200, China.

出版信息

Hereditas. 2025 May 9;162(1):72. doi: 10.1186/s41065-025-00442-3.

DOI:10.1186/s41065-025-00442-3
PMID:40346652
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12065329/
Abstract

BACKGROUND

The involvement of circ_RPPH1 in bladder urothelial carcinoma (BUC) remains unclear, as well as the underlying mechanism.

METHODS

Circ_RPPH1 levels in BUC cells and tissues were measured via RT-qPCR. Downregulation of circ_RPPH1 was assessed using colony formation, CCK-8, wound healing, and Transwell assays to evaluate proliferation, migration, and invasion. RIP and RNA pull-down confirmed circ_RPPH1 binding to EIF4A3, while immunoblotting analyzed EIF4A3 and EMT-related proteins.

RESULTS

High circ_RPPH1 levels in BUC correlated with tumor invasion depth. Its knockout suppressed proliferation, invasion, and EMT, while circ_RPPH1 overexpression reduced EIF4A3 binding to N-cadherin and Vimentin mRNA, promoting EMT.

CONCLUSION

Circ_RPPH1 promotes tumor growth and EMT in BUC by inhibiting EIF4A3-mediated mRNA regulation, activating the EIF4A3/N-cadherin/Vimentin pathway.

摘要

背景

circ_RPPH1在膀胱尿路上皮癌（BUC）中的作用及其潜在机制尚不清楚。

方法

通过RT-qPCR检测BUC细胞和组织中circ_RPPH1的水平。使用集落形成、CCK-8、伤口愈合和Transwell实验评估circ_RPPH1下调对增殖、迁移和侵袭的影响。RIP和RNA下拉实验证实circ_RPPH1与EIF4A3结合，免疫印迹分析EIF4A3和EMT相关蛋白。

结果

BUC中circ_RPPH1高表达与肿瘤浸润深度相关。敲除circ_RPPH1可抑制增殖、侵袭和EMT，而circ_RPPH1过表达减少EIF4A3与N-钙黏蛋白和波形蛋白mRNA的结合，促进EMT。

结论

circ_RPPH1通过抑制EIF4A3介导的mRNA调控，激活EIF4A3/N-钙黏蛋白/波形蛋白通路，促进BUC肿瘤生长和EMT。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/4b4f241ebb91/41065_2025_442_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/40ed93af7452/41065_2025_442_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/cc60589912f8/41065_2025_442_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/e8b5f7e50a90/41065_2025_442_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/49b33d3d1d9b/41065_2025_442_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/d4c2cc883de0/41065_2025_442_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/4b4f241ebb91/41065_2025_442_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/40ed93af7452/41065_2025_442_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/cc60589912f8/41065_2025_442_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/e8b5f7e50a90/41065_2025_442_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/49b33d3d1d9b/41065_2025_442_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/d4c2cc883de0/41065_2025_442_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5930/12065329/4b4f241ebb91/41065_2025_442_Fig6_HTML.jpg

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本文引用的文献

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Challenges and opportunities for circRNA identification and delivery.环状 RNA 的鉴定与递送面临的挑战与机遇。
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CircRNA and Ageing.环状RNA与衰老
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The oncogenic role of TFAP2A in bladder urothelial carcinoma via a novel long noncoding RNA TPRG1-AS1/DNMT3A/CRTAC1 axis.TFAP2A通过一种新型长链非编码RNA TPRG1-AS1/DNMT3A/CRTAC1轴在膀胱尿路上皮癌中的致癌作用。
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Circ_RPPH1 regulates glioma cell malignancy by binding to miR-627-5p/miR-663a to induce SDC1 expression.环状 RNA_RPPH1 通过结合 miR-627-5p/miR-663a 调控神经胶质瘤细胞恶性表型并诱导 SDC1 表达。
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