长期食用益生菌、燕麦和苹果对周围单核细胞中与胆汁酸、脂质、肠道肽及炎症相关基因表达的影响——CABALA研究结果

Impact of chronic consumption of probiotics, oats, and apples on expression of genes related to bile acids, lipid, gut peptides, and inflammation in peripheral monocular cells - findings from the CABALA study.

作者信息

Alzoufairi Shouq, Pushpass Rose-Anna G, Liu L, Lovegrove J A, Jackson K G

机构信息

Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, Institute for Cardiovascular and Metabolic Research, Institute for Food, Nutrition and Health, University of Reading, Harry Nursten Building, Reading, RG6 6DZ, UK.

出版信息

Eur J Nutr. 2025 May 10;64(4):176. doi: 10.1007/s00394-025-03694-x.

DOI:10.1007/s00394-025-03694-x

PMID:40347281

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12065741/

Abstract

PURPOSE

Chronic intakes of functional foods (probiotics, apples and oats) have been reported to have beneficial effects on hepatic lipid regulation and glycaemic control, but mechanistic human studies humans are limited. An ex-vivo study was performed to determine the chronic effects of probiotics, oats, and apples on the expression of genes related to markers of cardiometabolic health in peripheral blood monocular cells (PBMC).

METHODS

In this CABALA sub-study (n = 59/61, age: 52 ± 12y), blood PBMC were also isolated before and 8 weeks after the daily consumption of either a probiotic with bile salt hydrolase activity (Lactobacillus reuteri), porridge oats, Renetta Canada apples or a control. Relative PBMC mRNA gene expression was determined and correlations performed between the fold change in response to the functional interventions and change in cardiometabolic disease risk markers.

RESULTS

Relative to baseline, there was an upregulation in the PBMC TLR4 mRNA expression in the control compared with the probiotics and apples groups (p[Formula: see text]0.024). Moderate inverse correlations were found between the fold change in GPBAR1 mRNA expression and change in plasma total and secondary BAs, HMGCR and SREBF1 mRNA gene expressions and high-density lipoprotein-cholesterol, and SREBF1 and GIPR mRNA gene expressions and glucose. TLR4 and TNFSF14 mRNA gene expressions were associated with pro-inflammatory cytokines (p=0.05).

CONCLUSION

Probiotic and apples interventions attenuated the upregulation in PBMC TLR4 mRNA expression observed with the control. Correlations between fold change in mRNA gene expression and changes in cardiometabolic disease risk markers in response to the functional interventions were in agreement with previous studies.

CLINICAL TRIAL REGISTRY

The study was registered at clinical trials.gov (ref. NCT03369548).

摘要

目的

据报道，长期摄入功能性食品（益生菌、苹果和燕麦）对肝脏脂质调节和血糖控制具有有益作用，但相关人体机制研究有限。进行了一项体外研究，以确定益生菌、燕麦和苹果对人外周血单核细胞（PBMC）中心血管代谢健康标志物相关基因表达的长期影响。

方法

在这项CABALA子研究中（n = 59/61，年龄：52±12岁），在每日食用具有胆汁盐水解酶活性的益生菌（罗伊氏乳杆菌）、燕麦粥、加拿大瑞内特苹果或对照之前及之后8周，也分离了血液PBMC。测定了PBMC相对mRNA基因表达，并对功能性干预反应的倍数变化与心血管代谢疾病风险标志物变化之间进行了相关性分析。

结果

与基线相比，与益生菌和苹果组相比，对照组PBMC中TLR4 mRNA表达上调（p[公式：见正文]0.024）。在GPBAR1 mRNA表达的倍数变化与血浆总胆汁酸和次级胆汁酸变化、HMGCR和SREBF1 mRNA基因表达与高密度脂蛋白胆固醇之间，以及SREBF1和GIPR mRNA基因表达与葡萄糖之间发现了中度负相关。TLR4和TNFSF14 mRNA基因表达与促炎细胞因子相关（p = 0.05）。