Teves Cordova Abel Victor Isidro, Silva Meneses Júnior Nailson, Hinojosa Pedraza Fabricio, Oliveira de Amorim João Vitor, Priori Alcalde Murilo, Ricci Vivan Rodrigo, Bombarda de Andrade Flaviana, Hungaro Duarte Marco Antonio
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo- USP, Bauru,, Brazil.
School of Dentistry, UCAM Catholic University of Saint Anthony of Murcia , Murcia, Spain.
Lasers Med Sci. 2025 May 10;40(1):219. doi: 10.1007/s10103-025-04470-3.
The purpose of this research was to evaluate the impact of an 810 nm high-power diode laser on decontaminating both surface and intradentinal biofilm, as well as removing the smear layer. Forty human mandibular premolars were contaminated with a biofilm composed of Enterococcus faecalis and Streptococcus mutans. The teeth were divided into groups and treated with NaOCl 2.5% + PUI, saline solution + 810 nm laser, NaOCl 2.5% + PUI + 810 nm laser, and a control group. The samples were then evaluated using a confocal laser scanning microscope (CLSM). Another 40 teeth, prepared as dentin discs, were contaminated with the same biofilm to assess surface decontamination. Additionally, 80 bovine dentin discs with an induced smear layer, placed in the apical third of mesial root canals of 3D-printed teeth, underwent similar treatments. Pre- and post-treatment images were obtained using an environmental scanning electron microscope. Data were statistically analyzed with ANOVA test and Tukey's test, respectively. Intradentinal disinfection rates were 67,33% for PUI, 51,50% for the 810 nm laser, and 55,32% for the PUI + 810 nm laser, with no statistically significant differences (p > 0.05). Surface decontamination rates were 39,52%, 51,27%, and 45,20% for the respective groups, also without significant differences (p > 0.05). No significant differences were found regarding smear layer removal (p > 0.05). The 810 nm diode laser with saline achieved disinfection similar to 2.5% sodium hypochlorite with ultrasound. Combining laser and PUI didn't improve disinfection. None of the studied protocol removed the smear layer.
本研究的目的是评估810纳米高功率二极管激光对表面和牙本质内生物膜的去污效果，以及去除玷污层的效果。40颗人类下颌前磨牙被粪肠球菌和变形链球菌组成的生物膜污染。将牙齿分组，分别用2.5%次氯酸钠+被动超声冲洗（PUI）、生理盐水+810纳米激光、2.5%次氯酸钠+PUI+810纳米激光处理，并设对照组。然后使用共聚焦激光扫描显微镜（CLSM）对样本进行评估。另外40颗制备成牙本质盘的牙齿被相同的生物膜污染，以评估表面去污情况。此外，80个带有诱导玷污层的牛牙本质盘，放置在3D打印牙齿近中根管的根尖三分之一处，接受类似处理。使用环境扫描电子显微镜获取处理前后的图像。数据分别用方差分析（ANOVA）检验和Tukey检验进行统计学分析。PUI的牙本质内消毒率为67.33%，810纳米激光为51.50%，PUI+810纳米激光为55.32%，无统计学显著差异（p>0.05）。各处理组的表面去污率分别为39.52%、51.27%和45.20%，也无显著差异（p>0.05）。在玷污层去除方面未发现显著差异（p>0.05）。生理盐水联合810纳米二极管激光实现的消毒效果与2.5%次氯酸钠联合超声相似。激光与PUI联合使用并未提高消毒效果。所研究的方案均未去除玷污层。
