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二极管激光对人乳牙粪肠球菌的杀菌作用——一项体外研究

Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth-an in vitro study.

作者信息

Dai Shanshan, Xiao Gang, Dong Ning, Liu Fei, He Shuyang, Guo Qingyu

机构信息

Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Xi'an, 710004, China.

Department of Pediatric Dentistry, Hospital of Stomatology Xi'an Jiaotong University College of Medicine, Xi'an, 710004, Shaanxi, China.

出版信息

BMC Oral Health. 2018 Aug 31;18(1):154. doi: 10.1186/s12903-018-0611-6.

DOI:10.1186/s12903-018-0611-6
PMID:30165840
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6117880/
Abstract

BACKGROUND

In recent years, the diode laser (810 nm) has been used for root canal disinfection, which plays an important role in endodontic therapy. This study was undertaken to evaluate the disinfecting ability of a diode laser in experimentally infected root canals of primary teeth.

METHODS

Human retained mandibular primary anterior teeth without apical foramen resorption were selected and contaminated with Enterococcus faecalis for 21 days. The specimens were randomly divided into four groups: the negative group (no treatment), positive group (5.25% NaOCl), diode laser group (diode laser), and diode-NaOCl group (diode laser combined with NaOCl). The disinfecting abilities of the treatments were measured by the numbers of bacteria, scanning electron microscopy and confocal laser microscopy (live-dead staining).

RESULTS

Eighty teeth were selected. After irradiation and irrigation, the elimination of bacteria and the smear layer in the laser groups and positive group were significantly superior, compared with the negative group (p < 0.01). In the diode-NaOCl group, bacterial reduction reached nearly 100% on the surfaces of root canals; live bacteria were rarely observed, even in deeper dentinal tubules.

CONCLUSION

Use of a diode laser, especially in combination with NaOCl, was effective for disinfecting infected root canals of primary teeth.

摘要

背景

近年来,二极管激光(810纳米)已被用于根管消毒,这在牙髓治疗中发挥着重要作用。本研究旨在评估二极管激光对实验性感染的乳牙根管的消毒能力。

方法

选取无根尖孔吸收的人类滞留下颌乳前牙,用粪肠球菌污染21天。将标本随机分为四组:阴性组(不治疗)、阳性组(5.25%次氯酸钠)、二极管激光组(二极管激光)和二极管-次氯酸钠组(二极管激光联合次氯酸钠)。通过细菌数量、扫描电子显微镜和共聚焦激光显微镜(活死染色)来测量治疗的消毒能力。

结果

共选取80颗牙齿。照射和冲洗后,激光组和阳性组的细菌清除及玷污层清除情况明显优于阴性组(p < 0.01)。在二极管-次氯酸钠组中,根管表面细菌减少率接近100%;即使在较深的牙本质小管中也很少观察到活细菌。

结论

使用二极管激光,尤其是与次氯酸钠联合使用,对感染的乳牙根管消毒有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa3/6117880/0c6dc33d733b/12903_2018_611_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa3/6117880/7e638b0bb4ab/12903_2018_611_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa3/6117880/0c6dc33d733b/12903_2018_611_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa3/6117880/7e638b0bb4ab/12903_2018_611_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa3/6117880/0c6dc33d733b/12903_2018_611_Fig2_HTML.jpg

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