Mousanejad Elahe, Ezzatabadipour Massood, Baghalishahi Masoumeh, Shojaei Mohammad, Vafaei Shayan, Afgar Ali, Nematollahi-Mahani Seyed Noureddin
Kerman physiology Research Center, Institute of Neuropharmacology and Department of Anatomy, Kerman University of Medical Sciences, Kerman, Iran.
Mashhad University of Medical Sciences, Mashhad, Iran.
Lasers Med Sci. 2025 May 10;40(1):220. doi: 10.1007/s10103-025-04471-2.
Physical factors like LED light can influence cell behavior, impacting stem cell proliferation and differentiation. Stem cell-based therapies offer a promising alternative to heart transplantation for cardiac conditions like myocardial infarction. This study evaluated the effects of green LED light (530 nm) and a biochemical inducer cocktail (oxytocin and ascorbic acid) on human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) differentiation into cardiomyocyte-like cells. hAD-MSCs were treated with LED, the cocktail, and their combination. Cardiomyogenic markers NKX2.5, GATA4, cTnI, and CX43 were assessed via real-time PCR and western blotting. LED and the cocktail enhanced cardiac gene expression and protein synthesis, but their combination showed significantly higher effects, especially on day 7. This study demonstrates for the first time that green LED light combined with oxytocin and ascorbic acid can enhance hAD-MSC cardiac differentiation, supporting its potential in regenerative therapies following human studies.
诸如LED光等物理因素会影响细胞行为，影响干细胞的增殖和分化。基于干细胞的疗法为心肌梗死等心脏疾病的心脏移植提供了一种有前景的替代方案。本研究评估了绿色LED光（530纳米）和一种生化诱导剂混合物（催产素和抗坏血酸）对人脂肪组织来源的间充质干细胞（hAD-MSCs）分化为心肌样细胞的影响。用LED、混合物及其组合处理hAD-MSCs。通过实时PCR和蛋白质印迹法评估心肌生成标志物NKX2.5、GATA4、肌钙蛋白I（cTnI）和连接蛋白43（CX43）。LED和混合物增强了心脏基因表达和蛋白质合成，但它们的组合显示出显著更高的效果，尤其是在第7天。本研究首次证明，绿色LED光与催产素和抗坏血酸联合使用可增强hAD-MSCs向心脏细胞的分化，支持其在人体研究后的再生治疗中的潜力。
