Garg Prakrati, Ali Aaliya, Singh Sewa, Thakur Kanika, Raza Kaisar, Bhardwaj Bigul Yogeshver, Kulshrestha Saurabh, Negi Poonam
School of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Shoolini University, PO Box 9, Solan, Himachal Pradesh, 173229, India.
Center for Omics and Biodiversity Research, Shoolini University, PO Box 9, Solan, Himachal Pradesh, 173229, India.
Inflammopharmacology. 2025 May 10. doi: 10.1007/s10787-025-01740-y.
Moringa oleifera n-hexane (MO n-Hex) seed extract contains phytoconstituents such as behenic acid, quercetin, and kaempferol. These exhibit anti-inflammatory, antiallergic, and antioxidant properties and can alleviate atopic dermatitis (AD)-like symptoms.
This study aimed to develop and evaluate MO n-Hex seed extract-loaded microemulsion (ME) hydrogel to provide an effective, safe, non-steroidal, plant-based alternative to conventional therapies in the topical management of AD.
Optimized o/w ME was developed by building pseudo-ternary phase diagrams. Ex vivo skin penetration was determined by employing CLSM analysis. Further, skin compatibility, histological analysis, and pharmacodynamics were carried out using a DNCB-induced AD model in BALB/c mice.
The best o/w ME demonstrated nearly spherical globules with size < 50 nm, zeta potential - 28.83 ± 0.492, and pH value 5.433 ± 0.047. The in vivo efficacy revealed significant improvements in AD-like symptoms, healed ear skin lesions, and lowered IgE levels and inflammatory cytokiness (IL-4, IL-5, and IFN-γ). Further, histological analysis confirmed the restoration of skin structure, supporting the formulation's potential in skin barrier repair.
The study demonstrated that the MO n-Hex seed extract-loaded MEs were suitable for topical use with improved penetration to deeper layers of skin while showing safety and better skin compliance. The formulated MEs effectively modulated immune responses and restored skin structure and barrier functioning.
辣木籽正己烷（MO正己烷）提取物含有诸如山嵛酸、槲皮素和山奈酚等植物成分。这些成分具有抗炎、抗过敏和抗氧化特性，可缓解特应性皮炎（AD）样症状。
本研究旨在开发并评估负载辣木籽正己烷提取物的微乳（ME）水凝胶，以在AD的局部治疗中提供一种有效、安全、非甾体、基于植物的传统疗法替代方案。
通过构建伪三元相图来开发优化的油包水微乳。采用共聚焦激光扫描显微镜（CLSM）分析来测定体外皮肤渗透。此外，使用二硝基氯苯（DNCB）诱导的BALB/c小鼠AD模型进行皮肤相容性、组织学分析和药效学研究。
最佳的油包水微乳呈现出大小<50nm的近似球形颗粒，ζ电位为-28.83±0.492，pH值为5.433±0.047。体内疗效显示AD样症状有显著改善，耳部皮肤损伤愈合，IgE水平以及炎症细胞因子（IL-4、IL-5和IFN-γ）降低。此外，组织学分析证实了皮肤结构的恢复，支持该制剂在皮肤屏障修复方面的潜力。
该研究表明，负载辣木籽正己烷提取物的微乳适用于局部使用，具有改善的向皮肤深层的渗透能力，同时显示出安全性和更好的皮肤顺应性。所制备的微乳有效地调节了免疫反应并恢复了皮肤结构和屏障功能。
