通过光谱流式细胞术对临床外周血单个核细胞样本进行板内染色以进行高通量分析的方案。
Protocol for in-plate staining of clinical peripheral blood mononuclear cell samples for high-throughput analysis via spectral flow cytometry.
作者信息
Marciello Isabella, Feng Sonya, Gomez Diaz Ilana, Huezo Julia, Hodi F Stephen, Cimpean Maria, Baginska Joanna
机构信息
Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
出版信息
STAR Protoc. 2025 Jun 20;6(2):103775. doi: 10.1016/j.xpro.2025.103775. Epub 2025 May 9.
Clinical trial research relies on robust assays to monitor immune responses in patients. We present a protocol for optimization of in-plate staining for spectral flow cytometry tailored for high-throughput analysis of peripheral blood mononuclear cell (PBMC) samples from clinical research correlative studies. We describe steps for antibody titration, optimal in-plate staining, data acquisition on Sony ID7000 spectral cell analyzer, and validation of a flow cytometry assay.
临床试验研究依赖于可靠的检测方法来监测患者的免疫反应。我们提出了一种用于优化板内染色的方案，该方案适用于光谱流式细胞术，专为临床研究相关研究中对外周血单个核细胞（PBMC）样本进行高通量分析而定制。我们描述了抗体滴定、最佳板内染色、在索尼ID7000光谱细胞分析仪上进行数据采集以及流式细胞术检测验证的步骤。
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