Liu Jinzhi, Lin Zhiyuan, Wu Huanyou, Zhang Jianming, Wang Fujun, Wang Lu, Lu Shuliang, Gao Jing
Shanghai Frontiers Science Center of Advanced Textiles, College of Textiles, Donghua University, Shanghai 201620, China.
Hantech Medical Device Co. Ltd., No.288 Sanheng Road, Changhe Industrial Park, Cixi, Ningbo 315326, China.
Colloids Surf B Biointerfaces. 2025 Sep;253:114768. doi: 10.1016/j.colsurfb.2025.114768. Epub 2025 May 6.
Skin peripheral nerve injury repair still faces significant clinical challenges. Although nerve tissue engineering scaffolds show potential, issues such as limited functionality and low repair efficiency persist. This study developed a dual-regulation biomimetic composite nerve scaffold with oriented structure and conductive function to promote nerve injury repair. The structural layer was a chitosan (CS)/polycaprolactone (PCL) oriented nanofiber membrane, which could promote cell adhesion and induce directional growth of cells. The functional layer was a CS/sodium alginate (SA) ionic conductive hydrogel, which could enhance endogenous electric fields to promote cell proliferation and differentiation. The two layers were combined through physical crosslinking, avoiding the use of chemical adhesives and preserving the surface morphology of the nanofibrous membrane and the porous structure of the hydrogel. The biomimetic composite nerve scaffold exhibited layered degradability, excellent orientation, conductivity, and biocompatibility. Cell experiments indicated that the scaffold effectively induced directional migration, growth, and differentiation of cells and enhanced cell activity, thereby providing a favorable microenvironment for nerve regeneration. This study not only overcomes the limitation of functional singularity in traditional nerve scaffolds but also aligns with the forefront trend in tissue engineering toward multifunctional and biomimetic materials. It demonstrates great potential for treating complex conditions such as traumatic nerve defects and post-surgical nerve regeneration and has broad application prospects in the field of neural tissue engineering.
皮肤周围神经损伤修复仍面临重大临床挑战。尽管神经组织工程支架显示出潜力，但功能有限和修复效率低等问题依然存在。本研究开发了一种具有定向结构和导电功能的双调控仿生复合神经支架，以促进神经损伤修复。结构层是壳聚糖（CS）/聚己内酯（PCL）定向纳米纤维膜，可促进细胞黏附并诱导细胞定向生长。功能层是CS/海藻酸钠（SA）离子导电水凝胶，可增强内源性电场以促进细胞增殖和分化。两层通过物理交联结合，避免使用化学粘合剂，并保留了纳米纤维膜的表面形态和水凝胶的多孔结构。该仿生复合神经支架具有分层降解性、优异的定向性、导电性和生物相容性。细胞实验表明，该支架能有效诱导细胞定向迁移、生长和分化，并增强细胞活性，从而为神经再生提供有利的微环境。本研究不仅克服了传统神经支架功能单一的局限性，还顺应了组织工程向多功能和仿生材料发展的前沿趋势。它在治疗创伤性神经缺损和术后神经再生等复杂病症方面显示出巨大潜力，在神经组织工程领域具有广阔的应用前景。
