Fujiwara Naoko, Ueno Tsuyoshi, Yamazaki Tomohiro, Hirose Tetsuro
Graduate School of Frontier Biosciences, The University of Osaka, Suita 565-0871, Japan.
Graduate School of Frontier Biosciences, The University of Osaka, Suita 565-0871, Japan.
Biochim Biophys Acta Gen Subj. 2025 Jul;1869(8):130815. doi: 10.1016/j.bbagen.2025.130815. Epub 2025 May 8.
Architectural RNAs (arcRNAs) are long noncoding RNAs that serve as structural scaffolds for membraneless organelles (MLOs), facilitating cellular organization and dynamic responses to stimuli. Acting as blueprints for MLO assembly, arcRNAs recruit specific proteins and nucleic acids to establish and maintain the internal structure of MLOs while coordinating their spatial relationships with other organelles. This organized framework enables precise spatiotemporal regulation, allowing for targeted control of transcription, RNA processing, and cellular responses to stress. Notably, arcRNAs exhibit the "semi-extractable" feature, a property derived from their stable binding to cellular structures, making them partially resistant to conventional RNA extraction methods. This unique feature serves as a useful criterion for identifying novel arcRNAs, providing an opportunity to accelerate research in long noncoding RNAs and deepen our understanding of their functional roles in cellular processes.
架构RNA（arcRNAs）是长链非编码RNA，作为无膜细胞器（MLOs）的结构支架，促进细胞组织和对刺激的动态反应。作为MLO组装的蓝图，arcRNAs招募特定的蛋白质和核酸来建立和维持MLOs的内部结构，同时协调它们与其他细胞器的空间关系。这种有组织的框架实现了精确的时空调节，允许对转录、RNA加工和细胞应激反应进行靶向控制。值得注意的是，arcRNAs具有“半可提取”特性，这一特性源于它们与细胞结构的稳定结合，使它们对传统RNA提取方法具有部分抗性。这一独特特性是鉴定新型arcRNAs的有用标准，为加速长链非编码RNA的研究和加深我们对其在细胞过程中功能作用的理解提供了机会。
