Holland D L, East J, Gibson K H, Clayton E, Oldfield A
Prostaglandins. 1985 Jun;29(6):1021-9. doi: 10.1016/0090-6980(85)90225-4.
Barnacle egg hatching factor which is released into the mantle cavity where the egg masses are brooded and stimulates embryonic musculature resulting in hatching and liberation of the larvae into the sea has been isolated in a purified form from a common barnacle Balanus balanoides. Derivatised hatching factor has been analysed by GC-MS and identified as 10,11,12-trihydroxy-5,8,14,12-eicosatetraenoic acid, a novel eicosanoid probably formed from endogenous eicosapentaenoic acid (20:5w3). Hatching factor activity is the first specific physiological function to be established for this type of compound.
藤壶卵孵化因子是从常见的藤壶（鹅颈藤壶）中以纯化形式分离出来的，它被释放到育卵的外套腔中，刺激胚胎肌肉组织，导致幼虫孵化并释放到海中。衍生化的孵化因子已通过气相色谱-质谱联用仪（GC-MS）进行分析，并被鉴定为10,11,12-三羟基-5,8,14,12-二十碳四烯酸，这是一种可能由内源性二十碳五烯酸（20:5w3）形成的新型类二十烷酸。孵化因子活性是为这类化合物确定的首个特定生理功能。
